Tumor localization using fluorescence of indocyanine green (ICG) in rat models

Xingde Li, Bertrand Beauvoit, Renita White, Shoko Nioka, Britton Chance, Arjun G. Yodh

Research output: Chapter in Book/Report/Conference proceedingConference contribution

Abstract

The permeability of tumor blood vessels to contrast agents (Gd chelates) has formed the basis for MRI breast tumor identification. It is also believed that angiogenesis starts before further tumor growth. Here we report tumor detection and localization using the fluorescence of Indocyanine Green (ICG). ICG fluorescence is excited by CW NIR laser light with a central wavelength of 830 nm is recored as a function of time. Differential fluorescence signals were observed after a low-dose intraveneous injection of ICG aqueous solution in rat model experiments. The difference between the fluorescence signal from the tumor side and the fluorescence signal from the control side is detectable even when the tumor is very small. During the tumor exponential growth phase, the ratio of these two signals is approximately 2.5; the ratio of the initial ICG clearance velocity in the tumor leg to that in the control leg is about 3. New investigations on human subjects with breast tumors, or reoccurrence after breast tumors were removed, are underway, and preliminary differential fluorescence signals have been observed.

Original languageEnglish (US)
Title of host publicationOptical Tomography, Photon Migration, and Spectroscopy of Tissue and Model Media
Subtitle of host publicationTheory, Human Studies, and Instrumentation
EditorsBritton Chance, Robert R. Alfano
PublisherSPIE
Pages789-797
Number of pages9
ISBN (Electronic)9780819417367
DOIs
StatePublished - May 30 1995
Externally publishedYes
EventOptical Tomography, Photon Migration, and Spectroscopy of Tissue and Model Media: Theory, Human Studies, and Instrumentation - San Jose, United States
Duration: Feb 1 1995Feb 28 1995

Publication series

NameProceedings of SPIE - The International Society for Optical Engineering
Volume2389
ISSN (Print)0277-786X
ISSN (Electronic)1996-756X

Other

OtherOptical Tomography, Photon Migration, and Spectroscopy of Tissue and Model Media: Theory, Human Studies, and Instrumentation
Country/TerritoryUnited States
CitySan Jose
Period2/1/952/28/95

ASJC Scopus subject areas

  • Electronic, Optical and Magnetic Materials
  • Condensed Matter Physics
  • Computer Science Applications
  • Applied Mathematics
  • Electrical and Electronic Engineering

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