Tumor-infiltrating macrophages in post-transplant, relapsed classical Hodgkin lymphoma are donor-derived

Genevieve M. Crane, Mark A. Samols, Laura A. Morsberger, Raluca Yonescu, Michele L. Thiess, Denise A S Batista, Yi Ning, Kathleen H. Burns, Milena Vuica-Ross, Michael J. Borowitz, Christopher D. Gocke, Richard F. Ambinder, Amy S. Duffield

Research output: Contribution to journalArticle

Abstract

Tumor-associated inflammatory cells in classical Hodgkin lymphoma (CHL) typically outnumber the neoplastic Hodgkin/Reed-Sternberg (H/RS) cells. The composition of the inflammatory infiltrate, particularly the fraction of macrophages, has been associated with clinical behavior. Emerging work from animal models demonstrates that most tissue macrophages are maintained by a process of self-renewal under physiologic circumstances and certain inflammatory states, but the contribution from circulating monocytes may be increased in some disease states. This raises the question of the source of macrophages involved in human disease, particularly that of CHL. Patients with relapsed CHL following allogeneic bone marrow transplant (BMT) provide a unique opportunity to begin to address this issue. We identified 4 such patients in our archives. Through molecular chimerism and/or XY FISH studies, we demonstrated the DNA content in the post-BMT recurrent CHL was predominantly donor-derived, while the H/RS cells were derived from the patient. Where possible to evaluate, the cellular composition of the inflammatory infiltrate, including the percentage of macrophages, was similar to that of the original tumor. Our findings suggest that the H/RS cells themselves define the inflammatory environment. In addition, our results demonstrate that tumor-associated macrophages in CHL are predominantly derived from circulating monocytes rather than resident tissue macrophages. Given the association between tumor microenvironment and disease progression, a better understanding of macrophage recruitment to CHL may open new strategies for therapeutic intervention.

Original languageEnglish (US)
Article numbere0163559
JournalPLoS One
Volume11
Issue number9
DOIs
StatePublished - Sep 1 2016

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Hodgkin Disease
Macrophages
Transplants
Neoplasms
macrophages
Hodgkin disease
neoplasms
Contraception Behavior
cells
Iodamide
Supravalvular Aortic Stenosis
Reed-Sternberg Cells
bone marrow transplant
monocytes
Library Catalogs
African horse sickness virus
Bone
Monocytes
Bone Marrow
chimerism

ASJC Scopus subject areas

  • Medicine(all)
  • Biochemistry, Genetics and Molecular Biology(all)
  • Agricultural and Biological Sciences(all)

Cite this

Tumor-infiltrating macrophages in post-transplant, relapsed classical Hodgkin lymphoma are donor-derived. / Crane, Genevieve M.; Samols, Mark A.; Morsberger, Laura A.; Yonescu, Raluca; Thiess, Michele L.; Batista, Denise A S; Ning, Yi; Burns, Kathleen H.; Vuica-Ross, Milena; Borowitz, Michael J.; Gocke, Christopher D.; Ambinder, Richard F.; Duffield, Amy S.

In: PLoS One, Vol. 11, No. 9, e0163559, 01.09.2016.

Research output: Contribution to journalArticle

Crane GM, Samols MA, Morsberger LA, Yonescu R, Thiess ML, Batista DAS et al. Tumor-infiltrating macrophages in post-transplant, relapsed classical Hodgkin lymphoma are donor-derived. PLoS One. 2016 Sep 1;11(9). e0163559. Available from, DOI: 10.1371/journal.pone.0163559

Crane, Genevieve M.; Samols, Mark A.; Morsberger, Laura A.; Yonescu, Raluca; Thiess, Michele L.; Batista, Denise A S; Ning, Yi; Burns, Kathleen H.; Vuica-Ross, Milena; Borowitz, Michael J.; Gocke, Christopher D.; Ambinder, Richard F.; Duffield, Amy S. / Tumor-infiltrating macrophages in post-transplant, relapsed classical Hodgkin lymphoma are donor-derived.

In: PLoS One, Vol. 11, No. 9, e0163559, 01.09.2016.

Research output: Contribution to journalArticle

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