Tumor cell growth arrest caused by subchromosomal transferable DNA fragments from chromosome 11

Minora Koi, Laura A. Johnson, Linda M. Kalikin, Peter F R Little, Yusuke Nakamura, Andrew P Feinberg

Research output: Contribution to journalArticle

Abstract

A fundamental problem in the identification and isolation of tumor suppressor and other growth-inhibiting genes is the loss of power of genetic complementation at the subchromosomal level. A direct genetic strategy was developed to isolate subchromosomal transferable fragments (STFs) from any chromosome, each containing a selectable marker within the human DNA, that could be transferred to any mammalian cell. As a test of the method, several overlapping STFs from 11p15 were shown to cause in vitro growth arrest of rhabdomyosarcoma cells. This activity mapped between the β-globin and insulin genes.

Original languageEnglish (US)
Pages (from-to)361-364
Number of pages4
JournalScience
Volume260
Issue number5106
StatePublished - 1993
Externally publishedYes

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Chromosomes, Human, Pair 11
Globins
Rhabdomyosarcoma
DNA
Growth
Genes
Neoplasms
Chromosomes
Insulin
In Vitro Techniques

ASJC Scopus subject areas

  • General

Cite this

Koi, M., Johnson, L. A., Kalikin, L. M., Little, P. F. R., Nakamura, Y., & Feinberg, A. P. (1993). Tumor cell growth arrest caused by subchromosomal transferable DNA fragments from chromosome 11. Science, 260(5106), 361-364.

Tumor cell growth arrest caused by subchromosomal transferable DNA fragments from chromosome 11. / Koi, Minora; Johnson, Laura A.; Kalikin, Linda M.; Little, Peter F R; Nakamura, Yusuke; Feinberg, Andrew P.

In: Science, Vol. 260, No. 5106, 1993, p. 361-364.

Research output: Contribution to journalArticle

Koi, M, Johnson, LA, Kalikin, LM, Little, PFR, Nakamura, Y & Feinberg, AP 1993, 'Tumor cell growth arrest caused by subchromosomal transferable DNA fragments from chromosome 11', Science, vol. 260, no. 5106, pp. 361-364.
Koi M, Johnson LA, Kalikin LM, Little PFR, Nakamura Y, Feinberg AP. Tumor cell growth arrest caused by subchromosomal transferable DNA fragments from chromosome 11. Science. 1993;260(5106):361-364.
Koi, Minora ; Johnson, Laura A. ; Kalikin, Linda M. ; Little, Peter F R ; Nakamura, Yusuke ; Feinberg, Andrew P. / Tumor cell growth arrest caused by subchromosomal transferable DNA fragments from chromosome 11. In: Science. 1993 ; Vol. 260, No. 5106. pp. 361-364.
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