Tubulin polymerization modulates interleukin-2 receptor signal transduction in human T cells

Jens Goebel, Kathy Forrest, Marsha Wills-Karp, Thomas L. Roszman

Research output: Contribution to journalArticlepeer-review

Abstract

Few data exist on the modulation of cytokine receptor signaling by the actin or tubulin cytoskeleton. Therefore, we studied interleukin-2 receptor (IL-2R) signaling in phytohemagglutinine (PHA)-pretreated human T cells in the context of alterations in the cytoskeletal system induced by cytochalasin D (CyD), jasplaklinolide (Jas), taxol (Tax), or colchicine (Col). We found that changes in cytoskeletal tubulin polymerization altered the strength of several IL-2-triggered signals. Moreover, Tax-induced tubulin hyperpolymerization augmented the surface expression of the IL-2R β-chain and enhanced the association of the IL-2R γ-chain with cytoskeletal tubulin. The IL-2R β-chain, in turn, was constitutively associated with tubulin and, more weakly, actin. To exclude the possibility that these associations are artifacts caused by PHA, we confirmed them in T cells from TCR-transgenic DO11.10 mice stimulated with their nominal antigen. We conclude that altered polymerization of cytoskeletal components, especially tubulin, is accompanied by modulation of IL-2 signaling at the receptor level.

Original languageEnglish (US)
Pages (from-to)87-106
Number of pages20
JournalJournal of Receptors and Signal Transduction
Volume26
Issue number1-2
DOIs
StatePublished - Jan 1 2006
Externally publishedYes

Keywords

  • Actin and tubulin polymerization
  • Human T cells
  • Interleukin-2 receptor signal transduction

ASJC Scopus subject areas

  • Biochemistry
  • Molecular Biology
  • Cell Biology

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