Abstract
The distribution of glycylated tubulin has been analyzed in different populations of stable microtubules in a digenean flatworm, Echinostoma caproni (Platyhelminthes). Two cellular types, spermatozoa and ciliated excretory cells, have been analyzed by means of immunofluorescence, immunogold, and immunoblotting techniques using two monoclonal antibodies (mAbs), AXO 49, and TAP 952, specifically directed against differently glycylated isoforms of tubulin. The presence of glycylated tubulin in the two cell types was shown. However, the differential reactivities of TAP 952 and AXO 49 mAbs with the two axoneme types suggest a difference in their glycylation level. In addition, within a single cell, the spermatozoon, cortical microtubules underlying the flagellar membrane, and axonemal microtubules were shown to comprise different tubulin isoforms, the latter ones only being labelled with one of the antiglycylated tubulin mAbs, TAP 952. Similarly, the antiacetylated (6-11B-1) and polyglutamylated (GT335) tubulin mAbs decorated the two types of axonemal microtubules, but not the cortical ones. From these data, a subcellular sorting of posttranslationally modified tubulin isoforms within spermatozoa, on the one hand, and a cellular sorting of glycylated isoforms inside the whole organism, on the other hand, is demonstrated in the flatworm E. caproni. Last, a sequential occurrence of tubulin posttranslational modifications was observed in the course of spermiogenesis. Acetylation appears first, followed shortly by glutamylation; glycylation takes place at the extreme end of spermiogenesis and, specifically, in a proximo-distal process. Thus in agreement with, and extending other studies [Bre et al., 1996], glycylation appears to close the sequence of posttranslational events occurring in axonemal microtubules during spermiogenesis.
| Original language | English (US) |
|---|---|
| Pages (from-to) | 318-330 |
| Number of pages | 13 |
| Journal | Cell Motility and the Cytoskeleton |
| Volume | 39 |
| Issue number | 4 |
| DOIs | |
| State | Published - Apr 16 1998 |
| Externally published | Yes |
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Keywords
- Platyhelminthes
- Polyglycylation
- Posttranslational modifications
- Spermatozoa
- Stable microtubules
ASJC Scopus subject areas
- Cell Biology
Cite this
Tubulin polyglycylation in platyhelminthes : Diversity among stable microtubule networks and very late occurrence during spermiogenesis. / Iomini, Carlo; Bré, M. H.; Levilliers, Nicolette; Justine, Jean L.
In: Cell Motility and the Cytoskeleton, Vol. 39, No. 4, 16.04.1998, p. 318-330.Research output: Contribution to journal › Article
}
TY - JOUR
T1 - Tubulin polyglycylation in platyhelminthes
T2 - Diversity among stable microtubule networks and very late occurrence during spermiogenesis
AU - Iomini, Carlo
AU - Bré, M. H.
AU - Levilliers, Nicolette
AU - Justine, Jean L.
PY - 1998/4/16
Y1 - 1998/4/16
N2 - The distribution of glycylated tubulin has been analyzed in different populations of stable microtubules in a digenean flatworm, Echinostoma caproni (Platyhelminthes). Two cellular types, spermatozoa and ciliated excretory cells, have been analyzed by means of immunofluorescence, immunogold, and immunoblotting techniques using two monoclonal antibodies (mAbs), AXO 49, and TAP 952, specifically directed against differently glycylated isoforms of tubulin. The presence of glycylated tubulin in the two cell types was shown. However, the differential reactivities of TAP 952 and AXO 49 mAbs with the two axoneme types suggest a difference in their glycylation level. In addition, within a single cell, the spermatozoon, cortical microtubules underlying the flagellar membrane, and axonemal microtubules were shown to comprise different tubulin isoforms, the latter ones only being labelled with one of the antiglycylated tubulin mAbs, TAP 952. Similarly, the antiacetylated (6-11B-1) and polyglutamylated (GT335) tubulin mAbs decorated the two types of axonemal microtubules, but not the cortical ones. From these data, a subcellular sorting of posttranslationally modified tubulin isoforms within spermatozoa, on the one hand, and a cellular sorting of glycylated isoforms inside the whole organism, on the other hand, is demonstrated in the flatworm E. caproni. Last, a sequential occurrence of tubulin posttranslational modifications was observed in the course of spermiogenesis. Acetylation appears first, followed shortly by glutamylation; glycylation takes place at the extreme end of spermiogenesis and, specifically, in a proximo-distal process. Thus in agreement with, and extending other studies [Bre et al., 1996], glycylation appears to close the sequence of posttranslational events occurring in axonemal microtubules during spermiogenesis.
AB - The distribution of glycylated tubulin has been analyzed in different populations of stable microtubules in a digenean flatworm, Echinostoma caproni (Platyhelminthes). Two cellular types, spermatozoa and ciliated excretory cells, have been analyzed by means of immunofluorescence, immunogold, and immunoblotting techniques using two monoclonal antibodies (mAbs), AXO 49, and TAP 952, specifically directed against differently glycylated isoforms of tubulin. The presence of glycylated tubulin in the two cell types was shown. However, the differential reactivities of TAP 952 and AXO 49 mAbs with the two axoneme types suggest a difference in their glycylation level. In addition, within a single cell, the spermatozoon, cortical microtubules underlying the flagellar membrane, and axonemal microtubules were shown to comprise different tubulin isoforms, the latter ones only being labelled with one of the antiglycylated tubulin mAbs, TAP 952. Similarly, the antiacetylated (6-11B-1) and polyglutamylated (GT335) tubulin mAbs decorated the two types of axonemal microtubules, but not the cortical ones. From these data, a subcellular sorting of posttranslationally modified tubulin isoforms within spermatozoa, on the one hand, and a cellular sorting of glycylated isoforms inside the whole organism, on the other hand, is demonstrated in the flatworm E. caproni. Last, a sequential occurrence of tubulin posttranslational modifications was observed in the course of spermiogenesis. Acetylation appears first, followed shortly by glutamylation; glycylation takes place at the extreme end of spermiogenesis and, specifically, in a proximo-distal process. Thus in agreement with, and extending other studies [Bre et al., 1996], glycylation appears to close the sequence of posttranslational events occurring in axonemal microtubules during spermiogenesis.
KW - Platyhelminthes
KW - Polyglycylation
KW - Posttranslational modifications
KW - Spermatozoa
KW - Stable microtubules
UR - http://www.scopus.com/inward/record.url?scp=0031966304&partnerID=8YFLogxK
UR - http://www.scopus.com/inward/citedby.url?scp=0031966304&partnerID=8YFLogxK
U2 - 10.1002/(SICI)1097-0169(1998)39:4<318::AID-CM6>3.0.CO;2-Z
DO - 10.1002/(SICI)1097-0169(1998)39:4<318::AID-CM6>3.0.CO;2-Z
M3 - Article
C2 - 9556331
AN - SCOPUS:0031966304
VL - 39
SP - 318
EP - 330
JO - Cytoskeleton
JF - Cytoskeleton
SN - 1949-3584
IS - 4
ER -