Tubular epithelial cell and podocyte apoptosis with de novo sirolimus based immunosuppression in renal allograft recipients with DGF

R. Munivenkatappa, A. Haririan, J. C. Papadimitriou, C. B. Drachenberg, M. Dinits-Pensy, D. K. Klassen

Research output: Contribution to journalArticle

Abstract

Sirolimus is associated with prolonged delayed graft function (DGF) following renal transplantation and exacerbation of proteinuria. We assessed renal allograft biopsies from DGF patients treated with de novo sirolimus (n = 10) for renal tubular cell and podocyte apoptosis and expression of activated caspase-3, Bcl-2, and mTOR and compared them to biopsies from DGF patients not receiving sirolimus (n =15). Both groups received mycophenolate mofetil, prednisone and antibody induction. Apoptosis was assessed using terminal deoxynucleodidyl transferase mediated dUTP nick end labeling (TUNEL) staining. Caspase-3, Bcl-2, and mTOR expression were assessed by immunohistochemistry. Sirolimus treated patients had 334±69 TUNEL positive cells per 5 high power fields compared to 5.5±2.9 TUNEL positive cells in control patients (p

Original languageEnglish (US)
Pages (from-to)189-196
Number of pages8
JournalHistology and Histopathology
Volume25
Issue number2
Publication statusPublished - Feb 2010
Externally publishedYes

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Keywords

  • Apoptosis
  • Delayed graft function
  • Kidney
  • Podocyte
  • Sirolimus

ASJC Scopus subject areas

  • Histology
  • Pathology and Forensic Medicine

Cite this

Munivenkatappa, R., Haririan, A., Papadimitriou, J. C., Drachenberg, C. B., Dinits-Pensy, M., & Klassen, D. K. (2010). Tubular epithelial cell and podocyte apoptosis with de novo sirolimus based immunosuppression in renal allograft recipients with DGF. Histology and Histopathology, 25(2), 189-196.