tsunami, the Dictyostelium homolog of the Fused kinase, is required for polarization and chemotaxis

Linnan Tang, Jonathan Franca-Koh, Yuan Xiong, Mei Yu Chen, Yu Long, Ross M. Bickford, David A. Knecht, Pablo A Iglesias, Peter N Devreotes

Research output: Contribution to journalArticle

Abstract

In a forward genetic screen for chemotaxis mutants in Dictyostelium discoideum, we identified a loss-of-function mutation, designated tsunami, encoding a homolog of the Fused kinase. Cells lacking tsuA function could not effectively perform chemotaxis and were unable to become polarized or correctly orient pseudopods in chemotactic gradients. While tsuA- cells were able to couple receptor occupancy to phosphatidylinositol (3,4,5) trisphosphate (PIP3) production and actin polymerization, the PIP3 response was prolonged and basal F-actin levels were increased. Interestingly, TsuA localizes to the microtubule network and puncta mainly found at the cell periphery. Analysis of the gene uncovered a novel C-terminal domain that we designated the Tsunami Homology (TH) domain. Both the kinase domain and the TH domain are required to rescue the phenotypic defects of tsuA- cells. While kinase activity is not required for localization to microtubules, the TH domain is essential. Thus, localization of kinase activity to microtubules is critical for TsuA function. We propose that functions in association with the microtubule network may underlie the divergent roles of Fused kinase proteins in different organisms.

Original languageEnglish (US)
Pages (from-to)2278-2290
Number of pages13
JournalGenes & development
Volume22
Issue number16
DOIs
StatePublished - Aug 15 2008

Fingerprint

Tsunamis
Dictyostelium
Chemotaxis
Microtubules
Phosphotransferases
Actins
Pseudopodia
Polymerization
Protein Kinases
Mutation
Genes

Keywords

  • Chemotaxis
  • Dictyostelium
  • Fused
  • Polarization

ASJC Scopus subject areas

  • Genetics
  • Developmental Biology

Cite this

tsunami, the Dictyostelium homolog of the Fused kinase, is required for polarization and chemotaxis. / Tang, Linnan; Franca-Koh, Jonathan; Xiong, Yuan; Chen, Mei Yu; Long, Yu; Bickford, Ross M.; Knecht, David A.; Iglesias, Pablo A; Devreotes, Peter N.

In: Genes & development, Vol. 22, No. 16, 15.08.2008, p. 2278-2290.

Research output: Contribution to journalArticle

Tang, L, Franca-Koh, J, Xiong, Y, Chen, MY, Long, Y, Bickford, RM, Knecht, DA, Iglesias, PA & Devreotes, PN 2008, 'tsunami, the Dictyostelium homolog of the Fused kinase, is required for polarization and chemotaxis', Genes & development, vol. 22, no. 16, pp. 2278-2290. https://doi.org/10.1101/gad.1694508
Tang, Linnan ; Franca-Koh, Jonathan ; Xiong, Yuan ; Chen, Mei Yu ; Long, Yu ; Bickford, Ross M. ; Knecht, David A. ; Iglesias, Pablo A ; Devreotes, Peter N. / tsunami, the Dictyostelium homolog of the Fused kinase, is required for polarization and chemotaxis. In: Genes & development. 2008 ; Vol. 22, No. 16. pp. 2278-2290.
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