TsAg5, a Taenia solium cysticercus protein with a marginal trypsin-like activity in the diagnosis of human neurocysticercosis

Analiz Rueda, Cecilia Sifuentes, Robert H. Gilman, Andrés H. Gutiérrez, Ruby Piña, Nancy Chile, Sebastián Carrasco, Sandra Larson, Holger Mayta, Manuela Verástegui, Silvia Rodriguez, Marcel Gutiérrez-Correa, Héctor H. García, Patricia Sheen, Mirko Zimic

Research output: Contribution to journalArticlepeer-review

Abstract

Neurocysticercosis is an endemic parasitic disease caused by Taenia solium larva. Although the mechanism of infection is not completely understood, it is likely driven by proteolytic activity that degrades the intestinal wall to facilitate oncosphere penetration and further infection. We analyzed the publicly available T. solium EST/DNA library and identified two contigs comprising a full-length cDNA fragment very similar to Echinococcus granulosus Ag5 protein. The T. solium cDNA sequence included a proteolytic trypsin-like-domain in the C-terminal region, and a thrombospondin type-1 adherence-domain in the N-terminal region. Both the trypsin-like and adherence domains were expressed independently as recombinant proteins in bacterial systems. TsAg5 showed marginal trypsin-like activity and high sequence similarity to Ag5. The purified antigens were tested in a Western immunoblot assay to diagnose human neurocysticercosis. The sensitivity of the trypsin-like-domain was 96.36% in patients infected with extraparenchymal cysts, 75.44% in patients infected with multiple cysts, and 39.62% in patients with a single cyst. Specificity was 76.70%. The thrombospondin type-1 adherence-domain was not specific for neurocysticercosis.

Original languageEnglish (US)
Pages (from-to)115-119
Number of pages5
JournalMolecular and Biochemical Parasitology
Volume180
Issue number2
DOIs
StatePublished - Dec 2011

Keywords

  • Adherence
  • Cysticercus
  • Diagnostics
  • Oncosphere
  • Protease
  • Trypsin

ASJC Scopus subject areas

  • Parasitology
  • Molecular Biology

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