Tryptase 4, a New Member of the Chromosome 17 Family of Mouse Serine Proteases

Guang William Wong, Lixin Li, Mallur S. Madhusudhan, Steven A. Krilis, Michael F. Gurish, Marc E. Rothenberg, Andrej Šali, Richard L. Stevens

Research output: Contribution to journalArticle

Abstract

Genomic blot analysis raised the possibility that uncharacterized tryptase genes reside on chromosome 17 at the complex containing the three genes that encode mouse mast cell protease (mMCP) 6, mMCP-7, and transmembrane tryptase (mTMT). Probing of GenBank's expressed sequence tag data base with these three tryptase cDNAs resulted in the identification of an expressed sequence tag that encodes a portion of a novel mouse serine protease (now designated mouse tryptase 4 (mT4) because it is the fourth member of this family). 5′- and 3′-rapid amplification of cDNA ends approaches were carried out to deduce the nucleotide sequence of the full-length mT4 transcript. This information was then used to clone its ∼5.0-kilobase pair gene. Chromosome mapping analysis of its gene, sequence analysis of its transcript, and comparative protein structure modeling of its translated product revealed that mT4 is a new member of the chromosome 17 family of mouse tryptases. mT4 is 40-44% identical to mMCP-6, mMCP-7, and mTMT, and this new serine protease has all of the structural features of a functional tryptase. Moreover, mT4 is enzymatically active when expressed in insect cells. Due to its 17-mer hydrophobic domain at its C terminus, mT4 is a membrane-anchored tryptase more analogous to mTMT than the other members of its family. As assessed by RNA blot, reverse transcriptase-polymerase chain reaction, and/or in situ hybridization analysis, mT4 is expressed in interleukin-5-dependent mouse eosinophils, as well as in ovaries and testes. The observation that recombinant mT4 is preferentially retained in the endoplasmic reticulum of transiently transfected COS-7 cells suggests a convertase-like role for this integral membrane serine protease.

Original languageEnglish (US)
Pages (from-to)20648-20658
Number of pages11
JournalJournal of Biological Chemistry
Volume276
Issue number23
DOIs
StatePublished - Jun 8 2001
Externally publishedYes

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Tryptases
Chromosomes, Human, Pair 17
Serine Proteases
Chromosomes
Genes
Expressed Sequence Tags
Complementary DNA
Membranes
Chromosome Mapping
COS Cells
Polymerase chain reaction
RNA-Directed DNA Polymerase
Interleukin-5
Nucleic Acid Databases

ASJC Scopus subject areas

  • Biochemistry

Cite this

Wong, G. W., Li, L., Madhusudhan, M. S., Krilis, S. A., Gurish, M. F., Rothenberg, M. E., ... Stevens, R. L. (2001). Tryptase 4, a New Member of the Chromosome 17 Family of Mouse Serine Proteases. Journal of Biological Chemistry, 276(23), 20648-20658. https://doi.org/10.1074/jbc.M010422200

Tryptase 4, a New Member of the Chromosome 17 Family of Mouse Serine Proteases. / Wong, Guang William; Li, Lixin; Madhusudhan, Mallur S.; Krilis, Steven A.; Gurish, Michael F.; Rothenberg, Marc E.; Šali, Andrej; Stevens, Richard L.

In: Journal of Biological Chemistry, Vol. 276, No. 23, 08.06.2001, p. 20648-20658.

Research output: Contribution to journalArticle

Wong, GW, Li, L, Madhusudhan, MS, Krilis, SA, Gurish, MF, Rothenberg, ME, Šali, A & Stevens, RL 2001, 'Tryptase 4, a New Member of the Chromosome 17 Family of Mouse Serine Proteases', Journal of Biological Chemistry, vol. 276, no. 23, pp. 20648-20658. https://doi.org/10.1074/jbc.M010422200
Wong GW, Li L, Madhusudhan MS, Krilis SA, Gurish MF, Rothenberg ME et al. Tryptase 4, a New Member of the Chromosome 17 Family of Mouse Serine Proteases. Journal of Biological Chemistry. 2001 Jun 8;276(23):20648-20658. https://doi.org/10.1074/jbc.M010422200
Wong, Guang William ; Li, Lixin ; Madhusudhan, Mallur S. ; Krilis, Steven A. ; Gurish, Michael F. ; Rothenberg, Marc E. ; Šali, Andrej ; Stevens, Richard L. / Tryptase 4, a New Member of the Chromosome 17 Family of Mouse Serine Proteases. In: Journal of Biological Chemistry. 2001 ; Vol. 276, No. 23. pp. 20648-20658.
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