TY - JOUR
T1 - Trypsin and aminopeptidase gene expression is affected by age and food composition in Anopheles gambiae
AU - Lemos, Francisco J.A.
AU - Cornel, Anthonyon J.
AU - Jacobs-Lorena, Marcelo
PY - 1996/7/1
Y1 - 1996/7/1
N2 - The effects of age and food composition on the expression of trypsin and aminopeptidase genes in the Anopheles gambiae gut were investigated. No trypsin mRNA was detected in the gut of newly eclosed females, but this mRNA accumulated to relatively high levels within the first day of life. In contrast, low, but significant trypsin enzyme activity was observed in newly eclosed females. Subcellular fractionation experiments suggested that abdominal distention induces the secretion of the enzyme into the lumen. Blood, but not a protein-free meal, induced the accumulation of new trypsin mRNA and enzyme. Unlike trypsin, substantial aminopeptidase activity was detected in newly eclosed and in older, sugar fed mosquitoes. Moreover, the subcellular localization of aminopeptidase did not change appreciably with hind ingestion, and the early increase of enzyme activity was independent of food composition.
AB - The effects of age and food composition on the expression of trypsin and aminopeptidase genes in the Anopheles gambiae gut were investigated. No trypsin mRNA was detected in the gut of newly eclosed females, but this mRNA accumulated to relatively high levels within the first day of life. In contrast, low, but significant trypsin enzyme activity was observed in newly eclosed females. Subcellular fractionation experiments suggested that abdominal distention induces the secretion of the enzyme into the lumen. Blood, but not a protein-free meal, induced the accumulation of new trypsin mRNA and enzyme. Unlike trypsin, substantial aminopeptidase activity was detected in newly eclosed and in older, sugar fed mosquitoes. Moreover, the subcellular localization of aminopeptidase did not change appreciably with hind ingestion, and the early increase of enzyme activity was independent of food composition.
KW - Aminopeptidase
KW - Anopheles
KW - Digestion
KW - Trypsin
UR - http://www.scopus.com/inward/record.url?scp=0030186191&partnerID=8YFLogxK
UR - http://www.scopus.com/inward/citedby.url?scp=0030186191&partnerID=8YFLogxK
U2 - 10.1016/S0965-1748(96)00014-8
DO - 10.1016/S0965-1748(96)00014-8
M3 - Article
C2 - 8995788
AN - SCOPUS:0030186191
SN - 0965-1748
VL - 26
SP - 651
EP - 658
JO - Insect Biochemistry and Molecular Biology
JF - Insect Biochemistry and Molecular Biology
IS - 7
ER -