Trypanosoma brucei has two distinct mitochondrial DNA polymerase beta enzymes.

Tina T. Saxowsky, Gunjan Choudhary, Michele M. Klingbeil, Paul T. Englund

Research output: Contribution to journalArticle

Abstract

In higher eukaryotes, DNA polymerase (pol) beta resides in the nucleus and participates primarily in DNA repair. The DNA polymerase beta from the trypanosomatid Crithidia fasciculata, however, was the first mitochondrial enzyme of this type described. Upon searching the nearly completed genome data base of the related parasite Trypanosoma brucei, we discovered genes for two pol beta-like proteins. One is approximately 70% identical to the C. fasciculata pol beta and is likely the homolog of this enzyme. The other, although approximately 30% identical within the polymerase region, has unusual structural features including a short C-terminal tail and a long N-terminal extension rich in prolines, alanines, and lysines. Both proteins, when expressed recombinantly, are active as DNA polymerases and deoxyribose phosphate lyases, but their polymerase activity optima differ with respect to pH and KCl and MgCl2 concentrations. Remarkably, green fluorescent protein fusion proteins and immunofluorescence demonstrate that both are mitochondrial, but their locations with respect to the mitochondrial DNA (kinetoplast DNA network) in this organism are strikingly different.

Original languageEnglish (US)
Pages (from-to)49095-49101
Number of pages7
JournalJournal of Biological Chemistry
Volume278
Issue number49
StatePublished - Dec 5 2003

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DNA Polymerase beta
Trypanosoma brucei brucei
Crithidia fasciculata
Mitochondrial DNA
Enzymes
Genes
Kinetoplast DNA
Deoxyribose
Proteins
Magnesium Chloride
Lyases
DNA-Directed DNA Polymerase
Green Fluorescent Proteins
Eukaryota
Proline
DNA Repair
Alanine
Lysine
Fluorescent Antibody Technique
Tail

ASJC Scopus subject areas

  • Biochemistry

Cite this

Saxowsky, T. T., Choudhary, G., Klingbeil, M. M., & Englund, P. T. (2003). Trypanosoma brucei has two distinct mitochondrial DNA polymerase beta enzymes. Journal of Biological Chemistry, 278(49), 49095-49101.

Trypanosoma brucei has two distinct mitochondrial DNA polymerase beta enzymes. / Saxowsky, Tina T.; Choudhary, Gunjan; Klingbeil, Michele M.; Englund, Paul T.

In: Journal of Biological Chemistry, Vol. 278, No. 49, 05.12.2003, p. 49095-49101.

Research output: Contribution to journalArticle

Saxowsky, TT, Choudhary, G, Klingbeil, MM & Englund, PT 2003, 'Trypanosoma brucei has two distinct mitochondrial DNA polymerase beta enzymes.', Journal of Biological Chemistry, vol. 278, no. 49, pp. 49095-49101.
Saxowsky TT, Choudhary G, Klingbeil MM, Englund PT. Trypanosoma brucei has two distinct mitochondrial DNA polymerase beta enzymes. Journal of Biological Chemistry. 2003 Dec 5;278(49):49095-49101.
Saxowsky, Tina T. ; Choudhary, Gunjan ; Klingbeil, Michele M. ; Englund, Paul T. / Trypanosoma brucei has two distinct mitochondrial DNA polymerase beta enzymes. In: Journal of Biological Chemistry. 2003 ; Vol. 278, No. 49. pp. 49095-49101.
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