Trypanosoma brucei: Four tandemly linked genes for fatty acyl-CoA synthetases

David W. Jiang; Roxann Ingersoll; Peter J. Myler; Paul T. Englund

doi:10.1006/expr.2000.4547

Trypanosoma brucei: Four tandemly linked genes for fatty acyl-CoA synthetases

David W. Jiang, Roxann Ingersoll, Peter J. Myler, Paul T. Englund

Research output: Contribution to journal › Article › peer-review

3 Scopus citations

Abstract

We have cloned four acyl CoA synthetase (ACS) genes from Trypanosoma brucei strain 927. Each of these genes encodes a polypeptide about 78 kDa in size and all four contain the 'ACS signature motif.' Sequence alignments indicate that these proteins are 46%-95% identical in amino acid sequence. Interestingly, three of them share almost identical C-termini (about 215 amino acid residues). Southern blots suggest that these genes are present in a single copy, and Northern blots reveal that all four are expressed in both bloodstream and procyclic trypanosomes. (C) 2000 Academic Press.

Original language	English (US)
Pages (from-to)	16-22
Number of pages	7
Journal	Experimental Parasitology
Volume	96
Issue number	1
DOIs	https://doi.org/10.1006/expr.2000.4547
State	Published - 2000
Externally published	Yes

ASJC Scopus subject areas

Parasitology
Immunology
Infectious Diseases

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title = "Trypanosoma brucei: Four tandemly linked genes for fatty acyl-CoA synthetases",

abstract = "We have cloned four acyl CoA synthetase (ACS) genes from Trypanosoma brucei strain 927. Each of these genes encodes a polypeptide about 78 kDa in size and all four contain the 'ACS signature motif.' Sequence alignments indicate that these proteins are 46%-95% identical in amino acid sequence. Interestingly, three of them share almost identical C-termini (about 215 amino acid residues). Southern blots suggest that these genes are present in a single copy, and Northern blots reveal that all four are expressed in both bloodstream and procyclic trypanosomes. (C) 2000 Academic Press.",

author = "Jiang, {David W.} and Roxann Ingersoll and Myler, {Peter J.} and Englund, {Paul T.}",

note = "Funding Information: We thank Drs. Paul Watkins, Theresa Shapiro, Kim Paul, and Yasu Morita for critically reviewing the paper, Mark Drew and Viiu Klein for their help, and Jodie Franklin and Jennifer Meyers for sequencing the subcloned fragments. This study was supported by NIH Grant AI21334.",

year = "2000",

doi = "10.1006/expr.2000.4547",

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T2 - Four tandemly linked genes for fatty acyl-CoA synthetases

AU - Jiang, David W.

AU - Ingersoll, Roxann

AU - Myler, Peter J.

AU - Englund, Paul T.

N1 - Funding Information: We thank Drs. Paul Watkins, Theresa Shapiro, Kim Paul, and Yasu Morita for critically reviewing the paper, Mark Drew and Viiu Klein for their help, and Jodie Franklin and Jennifer Meyers for sequencing the subcloned fragments. This study was supported by NIH Grant AI21334.

PY - 2000

Y1 - 2000

N2 - We have cloned four acyl CoA synthetase (ACS) genes from Trypanosoma brucei strain 927. Each of these genes encodes a polypeptide about 78 kDa in size and all four contain the 'ACS signature motif.' Sequence alignments indicate that these proteins are 46%-95% identical in amino acid sequence. Interestingly, three of them share almost identical C-termini (about 215 amino acid residues). Southern blots suggest that these genes are present in a single copy, and Northern blots reveal that all four are expressed in both bloodstream and procyclic trypanosomes. (C) 2000 Academic Press.

AB - We have cloned four acyl CoA synthetase (ACS) genes from Trypanosoma brucei strain 927. Each of these genes encodes a polypeptide about 78 kDa in size and all four contain the 'ACS signature motif.' Sequence alignments indicate that these proteins are 46%-95% identical in amino acid sequence. Interestingly, three of them share almost identical C-termini (about 215 amino acid residues). Southern blots suggest that these genes are present in a single copy, and Northern blots reveal that all four are expressed in both bloodstream and procyclic trypanosomes. (C) 2000 Academic Press.

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Trypanosoma brucei: Four tandemly linked genes for fatty acyl-CoA synthetases

Abstract

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