Truncated PARP1 mediates ADP-ribosylation of RNA polymerase III for apoptosis

Qian Chen, Kai Ma, Xiuhua Liu, Shih Hsun Chen, Peng Li, Yonghao Yu, Anthony K.L. Leung, Xiaochun Yu

Research output: Contribution to journalArticlepeer-review


Caspase-mediated cleavage of PARP1 is a surrogate marker for apoptosis. However, the biological significance of PARP1 cleavage during apoptosis is still unclear. Here, using unbiased protein affinity purification, we show that truncated PARP1 (tPARP1) recognizes the RNA polymerase III (Pol III) complex in the cytosol. tPARP1 mono-ADP-ribosylates RNA Pol III in vitro and mediates ADP-ribosylation of RNA Pol III during poly(dA-dT)-stimulated apoptosis in cells. tPARP1-mediated activation of RNA Pol III facilitates IFN-β production and apoptosis. In contrast, suppression of PARP1 or expressing the non-cleavable form of PARP1 impairs these molecular events. Taken together, these studies reveal a novel biological role of tPARP1 during cytosolic DNA-induced apoptosis.

Original languageEnglish (US)
Article number3
JournalCell Discovery
Issue number1
StatePublished - Dec 2022

ASJC Scopus subject areas

  • Biochemistry
  • Molecular Biology
  • Genetics
  • Cell Biology


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