TRPV1 induction in airway vagal low-threshold mechanosensory neurons by allergen challenge and neurotrophic factors

Tina Marie Lieu, Allen C. Myers, Sonya Meeker, Bradley J Undem

Research output: Contribution to journalArticle

Abstract

We addressed the hypothesis that allergic inflammation in guinea pig airways leads to a phenotypic switch in vagal tracheal cough-causing, low-threshold mechanosensitive Aδ neurons, such that they begin expressing functional transient receptor potential vanilloid (TRPV1) channels. Guinea pigs were actively sensitized to ovalbumin (OVA) and beginning 21 days later exposed via aerosol to OVA daily for 3 days. Tracheal-specific neurons were identified in the nodose ganglion using retrograde tracing techniques. Tracheal specific neurons were isolated, and mRNA expression was evaluated at the single-neuron level using RT-PCR analysis. Electrophysiological studies have revealed that the vast majority of vagal nodose afferent nerves innervating the trachea are capsaicin-insensitive Aδ-fibers. Consistent with this, we found

Original languageEnglish (US)
JournalAmerican Journal of Physiology - Lung Cellular and Molecular Physiology
Volume302
Issue number9
DOIs
StatePublished - May 1 2012

Fingerprint

Nerve Growth Factors
Allergens
Neurons
Ovalbumin
Guinea Pigs
Nodose Ganglion
TRPV Cation Channels
Myelinated Nerve Fibers
Capsaicin
Trachea
Aerosols
Cough
Inflammation
Polymerase Chain Reaction
Messenger RNA

Keywords

  • Asthma
  • C-fiber
  • Capsaicin
  • Vagus

ASJC Scopus subject areas

  • Pulmonary and Respiratory Medicine
  • Physiology (medical)
  • Cell Biology
  • Physiology

Cite this

@article{eb90f67642914b58a9057d7224f9f062,
title = "TRPV1 induction in airway vagal low-threshold mechanosensory neurons by allergen challenge and neurotrophic factors",
abstract = "We addressed the hypothesis that allergic inflammation in guinea pig airways leads to a phenotypic switch in vagal tracheal cough-causing, low-threshold mechanosensitive Aδ neurons, such that they begin expressing functional transient receptor potential vanilloid (TRPV1) channels. Guinea pigs were actively sensitized to ovalbumin (OVA) and beginning 21 days later exposed via aerosol to OVA daily for 3 days. Tracheal-specific neurons were identified in the nodose ganglion using retrograde tracing techniques. Tracheal specific neurons were isolated, and mRNA expression was evaluated at the single-neuron level using RT-PCR analysis. Electrophysiological studies have revealed that the vast majority of vagal nodose afferent nerves innervating the trachea are capsaicin-insensitive Aδ-fibers. Consistent with this, we found",
keywords = "Asthma, C-fiber, Capsaicin, Vagus",
author = "Lieu, {Tina Marie} and Myers, {Allen C.} and Sonya Meeker and Undem, {Bradley J}",
year = "2012",
month = "5",
day = "1",
doi = "10.1152/ajplung.00366.2011",
language = "English (US)",
volume = "302",
journal = "American Journal of Physiology",
issn = "0363-6135",
publisher = "American Physiological Society",
number = "9",

}

TY - JOUR

T1 - TRPV1 induction in airway vagal low-threshold mechanosensory neurons by allergen challenge and neurotrophic factors

AU - Lieu, Tina Marie

AU - Myers, Allen C.

AU - Meeker, Sonya

AU - Undem, Bradley J

PY - 2012/5/1

Y1 - 2012/5/1

N2 - We addressed the hypothesis that allergic inflammation in guinea pig airways leads to a phenotypic switch in vagal tracheal cough-causing, low-threshold mechanosensitive Aδ neurons, such that they begin expressing functional transient receptor potential vanilloid (TRPV1) channels. Guinea pigs were actively sensitized to ovalbumin (OVA) and beginning 21 days later exposed via aerosol to OVA daily for 3 days. Tracheal-specific neurons were identified in the nodose ganglion using retrograde tracing techniques. Tracheal specific neurons were isolated, and mRNA expression was evaluated at the single-neuron level using RT-PCR analysis. Electrophysiological studies have revealed that the vast majority of vagal nodose afferent nerves innervating the trachea are capsaicin-insensitive Aδ-fibers. Consistent with this, we found

AB - We addressed the hypothesis that allergic inflammation in guinea pig airways leads to a phenotypic switch in vagal tracheal cough-causing, low-threshold mechanosensitive Aδ neurons, such that they begin expressing functional transient receptor potential vanilloid (TRPV1) channels. Guinea pigs were actively sensitized to ovalbumin (OVA) and beginning 21 days later exposed via aerosol to OVA daily for 3 days. Tracheal-specific neurons were identified in the nodose ganglion using retrograde tracing techniques. Tracheal specific neurons were isolated, and mRNA expression was evaluated at the single-neuron level using RT-PCR analysis. Electrophysiological studies have revealed that the vast majority of vagal nodose afferent nerves innervating the trachea are capsaicin-insensitive Aδ-fibers. Consistent with this, we found

KW - Asthma

KW - C-fiber

KW - Capsaicin

KW - Vagus

UR - http://www.scopus.com/inward/record.url?scp=84860519073&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=84860519073&partnerID=8YFLogxK

U2 - 10.1152/ajplung.00366.2011

DO - 10.1152/ajplung.00366.2011

M3 - Article

VL - 302

JO - American Journal of Physiology

JF - American Journal of Physiology

SN - 0363-6135

IS - 9

ER -