TY - JOUR
T1 - Tropism of Bunyaviruses
T2 - Evidence for a G1 Glycoprotein-Mediated Entry Pathway Common to the California Serogroup
AU - Pekosz, Andrew
AU - Griot, Christian
AU - Nathanson, Neal
AU - Gonzalez-Scarano, Francisco
N1 - Funding Information:
Supported by PHS Grant NS-20904 and Virology Training Grant AI-07325 (to A.P.). C.G. was the recipient of a research fellowship from the Schweizerische Stiftung für Medizinisch Biologische Stipendien and the Swiss National Foundation. We thank Dr. Susan Weiss, Department of Microbiology, University of Pennsylvania, for providing the EL-4 and L-cell lines, Drs. Roselyn Eisenberg and Gary Cohen for providing bac-gD2, Dr. Robert Shope for a number of viral strains, and all of the members of the Gonzalez/Nathanson laboratory for many valuable discussions.
PY - 1995/12/20
Y1 - 1995/12/20
N2 - The California serogroup is composed of antigenically and biologically related viruses within theBunyavirusgenus of the Bunyaviridae. We used a large panel of murine cells to study their tissue tropisms and found virtually identical patterns of viral replication among all of the members of this serogroup, in contrast to other members of the family (Bunyamwera, Cache Valley, and Punta Toro viruses). By analyzing the nonpermissive infections with both an RNA dot-blot and a virus binding assay, we determined that tropism for cultured cells was determined at the level of entry. A truncated soluble form of the La Crosse G1 glycoprotein (sG1) was expressed in a baculovirus system and, despite slight differences in glycosylation, was shown to resemble native G1 by immunoprecipitation with six monoclonal antibodies. sG1 bound to permissive but not to nonpermissive cell lines, as demonstrated by flow cytometry. The sG1 effectively blocked infection of permissive cell lines with all of the California serogroup viruses, but did not block infection of two other bunyaviruses. These results indicate that the California serogroup bunyaviruses share a common receptor on vertebrate cells which may differ from the receptor used by other Bunyaviridae and demonstrate that the G1 glycoprotein is the virus attachment protein. sG1 will be a useful reagent in the search for a putative receptor molecule.
AB - The California serogroup is composed of antigenically and biologically related viruses within theBunyavirusgenus of the Bunyaviridae. We used a large panel of murine cells to study their tissue tropisms and found virtually identical patterns of viral replication among all of the members of this serogroup, in contrast to other members of the family (Bunyamwera, Cache Valley, and Punta Toro viruses). By analyzing the nonpermissive infections with both an RNA dot-blot and a virus binding assay, we determined that tropism for cultured cells was determined at the level of entry. A truncated soluble form of the La Crosse G1 glycoprotein (sG1) was expressed in a baculovirus system and, despite slight differences in glycosylation, was shown to resemble native G1 by immunoprecipitation with six monoclonal antibodies. sG1 bound to permissive but not to nonpermissive cell lines, as demonstrated by flow cytometry. The sG1 effectively blocked infection of permissive cell lines with all of the California serogroup viruses, but did not block infection of two other bunyaviruses. These results indicate that the California serogroup bunyaviruses share a common receptor on vertebrate cells which may differ from the receptor used by other Bunyaviridae and demonstrate that the G1 glycoprotein is the virus attachment protein. sG1 will be a useful reagent in the search for a putative receptor molecule.
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U2 - 10.1006/viro.1995.0043
DO - 10.1006/viro.1995.0043
M3 - Article
C2 - 8553534
AN - SCOPUS:0029563569
SN - 0042-6822
VL - 214
SP - 339
EP - 348
JO - Virology
JF - Virology
IS - 2
M1 - 70043
ER -