Trna genes rapidly change in evolution to meet novel translational demands

Avihu H. Yona, Zohar Bloom-Ackermann, Idan Frumkin, Victor Hanson-Smith, Yoav Charpak-Amikam, Qinghua Feng, Jef D. Boeke, Orna Dahan, Yitzhak Pilpel

Research output: Contribution to journalArticle

Abstract

Changes in expression patterns may occur when organisms are presented with new environmental challenges, for example following migration or genetic changes. To elucidate the mechanisms by which the translational machinery adapts to such changes, we perturbed the tRNA pool of Saccharomyces cerevisiae by tRNA gene deletion. We then evolved the deletion strain and observed that the genetic adaptation was recurrently based on a strategic mutation that changed the anticodon of other tRNA genes to match that of the deleted one. Strikingly, a systematic search in hundreds of genomes revealed that anticodon mutations occur throughout the tree of life. We further show that the evolution of the tRNA pool also depends on the need to properly couple translation to protein folding. Together, our observations shed light on the evolution of the tRNA pool, demonstrating that mutation in the anticodons of tRNA genes is a common adaptive mechanism when meeting new translational demands.

Original languageEnglish (US)
Article numbere01339
JournaleLife
Volume2013
Issue number2
DOIs
StatePublished - Dec 20 2013

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Transfer RNA
Genes
Anticodon
Mutation
Protein folding
Protein Folding
Gene Deletion
Yeast
Machinery
Saccharomyces cerevisiae
Genome

ASJC Scopus subject areas

  • Biochemistry, Genetics and Molecular Biology(all)
  • Immunology and Microbiology(all)
  • Medicine(all)
  • Neuroscience(all)

Cite this

Yona, A. H., Bloom-Ackermann, Z., Frumkin, I., Hanson-Smith, V., Charpak-Amikam, Y., Feng, Q., ... Pilpel, Y. (2013). Trna genes rapidly change in evolution to meet novel translational demands. eLife, 2013(2), [e01339]. https://doi.org/10.7554/eLife.01339

Trna genes rapidly change in evolution to meet novel translational demands. / Yona, Avihu H.; Bloom-Ackermann, Zohar; Frumkin, Idan; Hanson-Smith, Victor; Charpak-Amikam, Yoav; Feng, Qinghua; Boeke, Jef D.; Dahan, Orna; Pilpel, Yitzhak.

In: eLife, Vol. 2013, No. 2, e01339, 20.12.2013.

Research output: Contribution to journalArticle

Yona, AH, Bloom-Ackermann, Z, Frumkin, I, Hanson-Smith, V, Charpak-Amikam, Y, Feng, Q, Boeke, JD, Dahan, O & Pilpel, Y 2013, 'Trna genes rapidly change in evolution to meet novel translational demands', eLife, vol. 2013, no. 2, e01339. https://doi.org/10.7554/eLife.01339
Yona AH, Bloom-Ackermann Z, Frumkin I, Hanson-Smith V, Charpak-Amikam Y, Feng Q et al. Trna genes rapidly change in evolution to meet novel translational demands. eLife. 2013 Dec 20;2013(2). e01339. https://doi.org/10.7554/eLife.01339
Yona, Avihu H. ; Bloom-Ackermann, Zohar ; Frumkin, Idan ; Hanson-Smith, Victor ; Charpak-Amikam, Yoav ; Feng, Qinghua ; Boeke, Jef D. ; Dahan, Orna ; Pilpel, Yitzhak. / Trna genes rapidly change in evolution to meet novel translational demands. In: eLife. 2013 ; Vol. 2013, No. 2.
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