Triple repetition time saturation transfer (TRiST) 31P spectroscopy for measuring human creatine kinase reaction kinetics

Research output: Contribution to journalArticle

Abstract

Human cardiac phosphorus MR saturation transfer experiments to quantify creatine kinase forward rate constants (kf) have previously been performed at 1.5 T. Such experiments could benefit from increased signal-to-noise ratio (SNR) and spectral resolution at 3 T. At 1.5 T, the four-angle saturation transfer method was applied with low-angle adiabatic pulses and surface coils. However, low-angle adiabatic pulses are potentially problematic above 1.5 T due to bandwidth limitations, power requirements, power deposition, and intrapulse spin-spin relaxation. For localized metabolite spin-lattice relaxation time (T1) measurements, a dual repetition time approach with adiabatic half-passage pulses was recently introduced to solve these problems at 3 T. Because the saturation transfer experiment requires a T1 measurement performed while one reacting moiety is saturated, we adapt the dual repetition time approach to measure kf using a triple repetition time saturation transfer (TRiST) method. A new pulsed saturation scheme with reduced sensitivity to static magnetic field inhomogeneity and compatibility with cardiac triggering is also presented. TRiST measurements of kf are validated in human calf muscle against conventional saturation transfer and found to agree within 3%. The first 3-T TRiST measurements of creatine kinase kf in the human calf (n = 6), chest muscle, and heart (n = 8) are 0.26 ± 0.04 s-1, 0.23 ± 0.03 s-1, and 0.32 ± 0.07 s-1, respectively, consistent with prior 1.5 T values.

Original languageEnglish (US)
Pages (from-to)1493-1501
Number of pages9
JournalMagnetic Resonance in Medicine
Volume63
Issue number6
DOIs
StatePublished - 2010

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Creatine Kinase
Spectrum Analysis
Signal-To-Noise Ratio
Magnetic Fields
Phosphorus
Myocardium
Thorax
Muscles

Keywords

  • 3 tesla
  • Creatine kinase
  • High-energy phosphate
  • Human heart
  • Metabolism
  • Reaction rate
  • Saturation pulses
  • Saturation transfer

ASJC Scopus subject areas

  • Radiology Nuclear Medicine and imaging
  • Medicine(all)

Cite this

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title = "Triple repetition time saturation transfer (TRiST) 31P spectroscopy for measuring human creatine kinase reaction kinetics",
abstract = "Human cardiac phosphorus MR saturation transfer experiments to quantify creatine kinase forward rate constants (kf) have previously been performed at 1.5 T. Such experiments could benefit from increased signal-to-noise ratio (SNR) and spectral resolution at 3 T. At 1.5 T, the four-angle saturation transfer method was applied with low-angle adiabatic pulses and surface coils. However, low-angle adiabatic pulses are potentially problematic above 1.5 T due to bandwidth limitations, power requirements, power deposition, and intrapulse spin-spin relaxation. For localized metabolite spin-lattice relaxation time (T1) measurements, a dual repetition time approach with adiabatic half-passage pulses was recently introduced to solve these problems at 3 T. Because the saturation transfer experiment requires a T1 measurement performed while one reacting moiety is saturated, we adapt the dual repetition time approach to measure kf using a triple repetition time saturation transfer (TRiST) method. A new pulsed saturation scheme with reduced sensitivity to static magnetic field inhomogeneity and compatibility with cardiac triggering is also presented. TRiST measurements of kf are validated in human calf muscle against conventional saturation transfer and found to agree within 3{\%}. The first 3-T TRiST measurements of creatine kinase kf in the human calf (n = 6), chest muscle, and heart (n = 8) are 0.26 ± 0.04 s-1, 0.23 ± 0.03 s-1, and 0.32 ± 0.07 s-1, respectively, consistent with prior 1.5 T values.",
keywords = "3 tesla, Creatine kinase, High-energy phosphate, Human heart, Metabolism, Reaction rate, Saturation pulses, Saturation transfer",
author = "Michael Schar and El-Sharkawy, {Abdel Monem M} and Weiss, {Robert George} and Bottomley, {Paul A}",
year = "2010",
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T1 - Triple repetition time saturation transfer (TRiST) 31P spectroscopy for measuring human creatine kinase reaction kinetics

AU - Schar, Michael

AU - El-Sharkawy, Abdel Monem M

AU - Weiss, Robert George

AU - Bottomley, Paul A

PY - 2010

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N2 - Human cardiac phosphorus MR saturation transfer experiments to quantify creatine kinase forward rate constants (kf) have previously been performed at 1.5 T. Such experiments could benefit from increased signal-to-noise ratio (SNR) and spectral resolution at 3 T. At 1.5 T, the four-angle saturation transfer method was applied with low-angle adiabatic pulses and surface coils. However, low-angle adiabatic pulses are potentially problematic above 1.5 T due to bandwidth limitations, power requirements, power deposition, and intrapulse spin-spin relaxation. For localized metabolite spin-lattice relaxation time (T1) measurements, a dual repetition time approach with adiabatic half-passage pulses was recently introduced to solve these problems at 3 T. Because the saturation transfer experiment requires a T1 measurement performed while one reacting moiety is saturated, we adapt the dual repetition time approach to measure kf using a triple repetition time saturation transfer (TRiST) method. A new pulsed saturation scheme with reduced sensitivity to static magnetic field inhomogeneity and compatibility with cardiac triggering is also presented. TRiST measurements of kf are validated in human calf muscle against conventional saturation transfer and found to agree within 3%. The first 3-T TRiST measurements of creatine kinase kf in the human calf (n = 6), chest muscle, and heart (n = 8) are 0.26 ± 0.04 s-1, 0.23 ± 0.03 s-1, and 0.32 ± 0.07 s-1, respectively, consistent with prior 1.5 T values.

AB - Human cardiac phosphorus MR saturation transfer experiments to quantify creatine kinase forward rate constants (kf) have previously been performed at 1.5 T. Such experiments could benefit from increased signal-to-noise ratio (SNR) and spectral resolution at 3 T. At 1.5 T, the four-angle saturation transfer method was applied with low-angle adiabatic pulses and surface coils. However, low-angle adiabatic pulses are potentially problematic above 1.5 T due to bandwidth limitations, power requirements, power deposition, and intrapulse spin-spin relaxation. For localized metabolite spin-lattice relaxation time (T1) measurements, a dual repetition time approach with adiabatic half-passage pulses was recently introduced to solve these problems at 3 T. Because the saturation transfer experiment requires a T1 measurement performed while one reacting moiety is saturated, we adapt the dual repetition time approach to measure kf using a triple repetition time saturation transfer (TRiST) method. A new pulsed saturation scheme with reduced sensitivity to static magnetic field inhomogeneity and compatibility with cardiac triggering is also presented. TRiST measurements of kf are validated in human calf muscle against conventional saturation transfer and found to agree within 3%. The first 3-T TRiST measurements of creatine kinase kf in the human calf (n = 6), chest muscle, and heart (n = 8) are 0.26 ± 0.04 s-1, 0.23 ± 0.03 s-1, and 0.32 ± 0.07 s-1, respectively, consistent with prior 1.5 T values.

KW - 3 tesla

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KW - Human heart

KW - Metabolism

KW - Reaction rate

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