TY - JOUR
T1 - Triosephosphate Isomerase Gene Characterization and Potential Zoonotic Transmission of Giardia duodenalis
AU - Sulaiman, Irshad M.
AU - Fayer, Ronald
AU - Bern, Caryn
AU - Gilman, Robert H.
AU - Trout, James M.
AU - Schantz, Peter M.
AU - Das, Pradeep
AU - Lal, Altaf A.
AU - Xiao, Lihua
PY - 2003/11
Y1 - 2003/11
N2 - To address the source of infection in humans and public health importance of Giardia duodenalis parasites from animals, nucleotide sequences of the triosephosphate isomerase (TPI) gene were generated for 37 human isolates, 15 dog isolates, 8 muskrat isolates, 7 isolates each from cattle and beavers, and 1 isolate each from a rat and a rabbit. Distinct genotypes were found in humans, cattle, beavers, dogs, muskrats, and rats. TPI and small subunit ribosomal RNA (SSU rRNA) gene sequences of G. microti from muskrats were also generated and analyzed. Phylogenetic analysis on the TPI sequences confirmed the formation of distinct groups. Nevertheless, a major group (assemblage B) contained most of the human and muskrat isolates, all beaver isolates, and the rabbit isolate. These data confirm that G. duodenalis from certain animals can potentially infect humans and should be useful in the detection, differentiation, and taxonomy of Giardia spp.
AB - To address the source of infection in humans and public health importance of Giardia duodenalis parasites from animals, nucleotide sequences of the triosephosphate isomerase (TPI) gene were generated for 37 human isolates, 15 dog isolates, 8 muskrat isolates, 7 isolates each from cattle and beavers, and 1 isolate each from a rat and a rabbit. Distinct genotypes were found in humans, cattle, beavers, dogs, muskrats, and rats. TPI and small subunit ribosomal RNA (SSU rRNA) gene sequences of G. microti from muskrats were also generated and analyzed. Phylogenetic analysis on the TPI sequences confirmed the formation of distinct groups. Nevertheless, a major group (assemblage B) contained most of the human and muskrat isolates, all beaver isolates, and the rabbit isolate. These data confirm that G. duodenalis from certain animals can potentially infect humans and should be useful in the detection, differentiation, and taxonomy of Giardia spp.
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U2 - 10.3201/eid0911.030084
DO - 10.3201/eid0911.030084
M3 - Article
C2 - 14718089
AN - SCOPUS:0242386555
SN - 1080-6040
VL - 9
SP - 1444
EP - 1452
JO - Emerging infectious diseases
JF - Emerging infectious diseases
IS - 11
ER -