Trimethoprim resistance gene in Shigella dysenteriae 1 isolates obtained from widely scattered locations of Asia

K. Haider, A. Chatkaeomorakot, B. A. Kay, K. A. Talukder, D. N. Taylor, P. Echeverria, D. A. Sack

Research output: Contribution to journalArticle

Abstract

Trimethoprim-resistance genes of Shigella dysenteriae 1 strains, isolated from a different location of six different countries of Asia over a 5-year period were characterized by using three different dihydrofolate reductase (DHFR) gene probes. The trimethoprim-resistant (TMPR) strains hybridized only with the type I DHFR gene probe by colony hybridization. None of the strains hybridized with types II and III DHFR gene probes. Southern blot experiments using plasmid DNA extracted from these resistant strains indicated that the type I DHFR genes were either on a 20 MDa plasmid or might be located on the chromosome. None of the other plasmids present in S. dyysenteriae 1 strains hybridized with the probe. This indicates that the TMP resistance in these S. dysenteriae 1 strains are mediated by type I DHFR enzyme, and there may be transposition of this type I DHFR gene occurs between the 20 MDa plasmid and the chromosome in this serotype of shigella.

Original languageEnglish (US)
Pages (from-to)219-228
Number of pages10
JournalEpidemiology and infection
Volume104
Issue number2
DOIs
StatePublished - Apr 1990

ASJC Scopus subject areas

  • Epidemiology
  • Infectious Diseases

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