Triggering of RNA interference with RNA-RNA, RNA-DNA, and DNA-RNA nanoparticles

Kirill A. Afonin; Mathias Viard; Ioannis Kagiampakis; Christopher L. Case; Marina A. Dobrovolskaia; Jen Hofmann; Ashlee Vrzak; Maria Kireeva; Wojciech K. Kasprzak; Vineet N. Kewalramani; Bruce A. Shapiro

doi:10.1021/nn504508s

Triggering of RNA interference with RNA-RNA, RNA-DNA, and DNA-RNA nanoparticles

Kirill A. Afonin, Mathias Viard, Ioannis Kagiampakis, Christopher L. Case, Marina A. Dobrovolskaia, Jen Hofmann, Ashlee Vrzak, Maria Kireeva, Wojciech K. Kasprzak, Vineet N. Kewalramani, Bruce A. Shapiro

School of Medicine

Research output: Contribution to journal › Article › peer-review

69 Scopus citations

Abstract

Control over cellular delivery of different functionalities and their synchronized activation is a challenging task. We report several RNA and RNA/DNA-based nanoparticles designed to conditionally activate the RNA interference in various human cells. These nanoparticles allow precise control over their formulation, stability in blood serum, and activation of multiple functionalities. Importantly, interferon and pro-inflammatory cytokine activation assays indicate the significantly lower responses for DNA nanoparticles compared to the RNA counterparts, suggesting greater potential of these molecules for therapeutic use.

Original language	English (US)
Pages (from-to)	251-259
Number of pages	9
Journal	ACS Nano
Volume	9
Issue number	1
DOIs	https://doi.org/10.1021/nn504508s
State	Published - Jan 27 2015

Keywords

FRET
RNA and DNA nanoparticles
RNA and DNA nanotechnology
RNA-DNA hybrids reassociation
RNAi

ASJC Scopus subject areas

General Materials Science
General Engineering
General Physics and Astronomy

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10.1021/nn504508s

Cite this

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title = "Triggering of RNA interference with RNA-RNA, RNA-DNA, and DNA-RNA nanoparticles",

abstract = "Control over cellular delivery of different functionalities and their synchronized activation is a challenging task. We report several RNA and RNA/DNA-based nanoparticles designed to conditionally activate the RNA interference in various human cells. These nanoparticles allow precise control over their formulation, stability in blood serum, and activation of multiple functionalities. Importantly, interferon and pro-inflammatory cytokine activation assays indicate the significantly lower responses for DNA nanoparticles compared to the RNA counterparts, suggesting greater potential of these molecules for therapeutic use.",

keywords = "FRET, RNA and DNA nanoparticles, RNA and DNA nanotechnology, RNA-DNA hybrids reassociation, RNAi",

author = "Afonin, {Kirill A.} and Mathias Viard and Ioannis Kagiampakis and Case, {Christopher L.} and Dobrovolskaia, {Marina A.} and Jen Hofmann and Ashlee Vrzak and Maria Kireeva and Kasprzak, {Wojciech K.} and Kewalramani, {Vineet N.} and Shapiro, {Bruce A.}",

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T1 - Triggering of RNA interference with RNA-RNA, RNA-DNA, and DNA-RNA nanoparticles

AU - Afonin, Kirill A.

AU - Viard, Mathias

AU - Kagiampakis, Ioannis

AU - Case, Christopher L.

AU - Dobrovolskaia, Marina A.

AU - Hofmann, Jen

AU - Vrzak, Ashlee

AU - Kireeva, Maria

AU - Kasprzak, Wojciech K.

AU - Kewalramani, Vineet N.

AU - Shapiro, Bruce A.

PY - 2015/1/27

Y1 - 2015/1/27

N2 - Control over cellular delivery of different functionalities and their synchronized activation is a challenging task. We report several RNA and RNA/DNA-based nanoparticles designed to conditionally activate the RNA interference in various human cells. These nanoparticles allow precise control over their formulation, stability in blood serum, and activation of multiple functionalities. Importantly, interferon and pro-inflammatory cytokine activation assays indicate the significantly lower responses for DNA nanoparticles compared to the RNA counterparts, suggesting greater potential of these molecules for therapeutic use.

AB - Control over cellular delivery of different functionalities and their synchronized activation is a challenging task. We report several RNA and RNA/DNA-based nanoparticles designed to conditionally activate the RNA interference in various human cells. These nanoparticles allow precise control over their formulation, stability in blood serum, and activation of multiple functionalities. Importantly, interferon and pro-inflammatory cytokine activation assays indicate the significantly lower responses for DNA nanoparticles compared to the RNA counterparts, suggesting greater potential of these molecules for therapeutic use.

KW - FRET

KW - RNA and DNA nanoparticles

KW - RNA and DNA nanotechnology

KW - RNA-DNA hybrids reassociation

KW - RNAi

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Triggering of RNA interference with RNA-RNA, RNA-DNA, and DNA-RNA nanoparticles

Abstract

Keywords

ASJC Scopus subject areas

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