Tricyclic antidepressant radioreceptor assay

Robert B. Innis, Larry Tune, Robert Rock, J Raymond Depaulo, David C. U'Prichard, Solomon H Snyder

Research output: Contribution to journalArticle

Abstract

A radioreceptor assay for tricyclic antideressants described here is based on the ability of these drugs to compete with [3H]-3-quinuclidinyl benzatile (3H-QNB) for binding to muscarinic cholinergic receptors in rat brain membranes. The assay is sensitive, in that it can detect, for example, 2 ng/ml nortriptyline in plasma. Seven plasma samples from depressed patients treated with notriptyline were assayed with the radioreceptor and gas liquid chromatographic methods, and the results for these two methods were almost identical. This assay should be used continously, if at all, in patients treated with other drugs that have potent anticholinergic effects.

Original languageEnglish (US)
Pages (from-to)473-477
Number of pages5
JournalEuropean Journal of Pharmacology
Volume58
Issue number4
DOIs
StatePublished - Oct 15 1979

Fingerprint

Radioligand Assay
Tricyclic Antidepressive Agents
Nortriptyline
Cholinergic Antagonists
Cholinergic Receptors
Muscarinic Receptors
Pharmaceutical Preparations
Gases
Membranes
Brain

Keywords

  • Anticholinergic
  • QNB displacement
  • Radioreceptor assay
  • Tricyclic antidepressant

ASJC Scopus subject areas

  • Cellular and Molecular Neuroscience
  • Pharmacology

Cite this

Tricyclic antidepressant radioreceptor assay. / Innis, Robert B.; Tune, Larry; Rock, Robert; Depaulo, J Raymond; U'Prichard, David C.; Snyder, Solomon H.

In: European Journal of Pharmacology, Vol. 58, No. 4, 15.10.1979, p. 473-477.

Research output: Contribution to journalArticle

Innis, Robert B. ; Tune, Larry ; Rock, Robert ; Depaulo, J Raymond ; U'Prichard, David C. ; Snyder, Solomon H. / Tricyclic antidepressant radioreceptor assay. In: European Journal of Pharmacology. 1979 ; Vol. 58, No. 4. pp. 473-477.
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