Tricyclic antidepressant determination in human plasma by gas-liquid chromatography using nitrogen-phosphorous detection: Application to single-dose pharmacokinetic studies

Darrell R. Abernethy, David J. Greenblatt, Richard I. Shader

Research output: Contribution to journalArticlepeer-review

Abstract

A method with adequate sensitivity (to 0.50-0.75 ng/ml) for single-dose pharmacokinetic studies which utilizes gas-liquid chromatography with nitrogen-phosphorous detection for tricyclic antidepressants (imipramine, desipramine, amitriptyline, nortriptyline, and doxepin) is described. A basic (pH 13) extraction with acid back-wash and subsequent basic reextraction is used for plasma sample preparation. Standard curves, using clomipramine as the internal standard, are linear for concentrations from 1 to 200 ng/ml for all tricyclic antidepressants. Applicability of the method is demonstrated by a pharmacokinetic study in a normal volunteer who received 12.5 mg imipramine hydrochloride i.v.

Original languageEnglish (US)
Pages (from-to)57-63
Number of pages7
JournalPharmacology
Volume23
Issue number2
DOIs
StatePublished - Jan 1 1981

Keywords

  • Gas chromatography
  • Nitrogen detection
  • Single-dose pharmacokinetics
  • Tricyclic antideprcssants

ASJC Scopus subject areas

  • Pharmacology

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