Trichomonas vaginalis polymerase chain reaction compared with standard diagnostic and therapeutic protocols for detection and treatment of vaginal trichornoniasis

Karen A. Wendel, Emily J. Erbelding, Charlotte A Gaydos, Anne Marie Rompalo

Research output: Contribution to journalArticle

Abstract

Wet preparation has limited sensitivity for diagnosis of Trichomonas vaginalis (TV) infection. An observational study of 337 women was conducted to evaluate a new polymerase chain reaction (PCR) test for TV. The sensitivities of wet preparation and TV culture were 52% (95% confidence interval [CI], 41-62) and 78% (95% CI, 69-86), respectively. TV PCR had a sensitivity of 84% (95% CI, 75-90) and a specificity of 94% (95% CI, 90-97). Metronidazole was provided to 67 (69%) of 97 women with TV because of TV on wet preparation, exposure to TV, or a diagnosis of bacterial vaginosis or pelvic inflammatory disease; however, if TV PCR had been used for diagnosis, 81 (84%) of 97 women with TV would have been treated (P = .02). TV is significantly undertreated using standard algorithms for metronidazole therapy. Given the association of trichomoniasis with perinatal morbidity and HIV transmission, women in high-risk groups may benefit from TV PCR.

Original languageEnglish (US)
Pages (from-to)576-580
Number of pages5
JournalClinical Infectious Diseases
Volume35
Issue number5
DOIs
StatePublished - Sep 1 2002

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Trichomonas vaginalis
Clinical Protocols
Polymerase Chain Reaction
Therapeutics
Confidence Intervals
Metronidazole
Trichomonas Infections
Bacterial Vaginosis
Pelvic Inflammatory Disease
Observational Studies
HIV

ASJC Scopus subject areas

  • Immunology

Cite this

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title = "Trichomonas vaginalis polymerase chain reaction compared with standard diagnostic and therapeutic protocols for detection and treatment of vaginal trichornoniasis",
abstract = "Wet preparation has limited sensitivity for diagnosis of Trichomonas vaginalis (TV) infection. An observational study of 337 women was conducted to evaluate a new polymerase chain reaction (PCR) test for TV. The sensitivities of wet preparation and TV culture were 52{\%} (95{\%} confidence interval [CI], 41-62) and 78{\%} (95{\%} CI, 69-86), respectively. TV PCR had a sensitivity of 84{\%} (95{\%} CI, 75-90) and a specificity of 94{\%} (95{\%} CI, 90-97). Metronidazole was provided to 67 (69{\%}) of 97 women with TV because of TV on wet preparation, exposure to TV, or a diagnosis of bacterial vaginosis or pelvic inflammatory disease; however, if TV PCR had been used for diagnosis, 81 (84{\%}) of 97 women with TV would have been treated (P = .02). TV is significantly undertreated using standard algorithms for metronidazole therapy. Given the association of trichomoniasis with perinatal morbidity and HIV transmission, women in high-risk groups may benefit from TV PCR.",
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