@article{aa363f0c4b244a5f94cdffc849e64ebf,
title = "Trichomonas vaginalis: Evaluating capsid proteins of dsRNA viruses and the dsRNA virus within patients attending a sexually transmitted disease clinic",
abstract = "Some isolates of Trichomonas vaginalis, the number one, non-viral sexually transmitted disease agent, are infected with one or several distinct double stranded (ds)-RNA virus. Immune rabbit anti-capsid serum (IRS) reacted with the capsid protein of purified dsRNA virus of a subset of the virus-infected T. vaginalis isolates. A monoclonal antibody (mAb) that recognized the capsid protein reactive with the IRS was generated. Analysis of the virus capsid protein of virus-infected isolates by probing nitrocellulose blots with mAb revealed diversity among immunoreactivity and in the size of the reactive capsid protein. Despite difficulties in visualizing virus within parasites by cross-section electron microscopy, gold-conjugated mAb readily labeled the cytoplasm of virus-positive trichomonads. Finally and importantly, isolates infecting patients attending an STD clinic, 75% of which were virus-positive isolates, had capsid protein of the same size detected by mAb present in all dsRNA viruses.",
keywords = "Patients, Sexually transmitted diseases, Trichomonas vaginalis, Urogenital, Vaginal, dsRNA virus",
author = "Alderete, {J. F.} and Wendel, {K. A.} and Rompalo, {A. M.} and Erbelding, {E. J.} and M. Benchimol and Chang, {T. H.}",
note = "Funding Information: We are grateful to Dr. J.-H. Tai of the Academia Sinica of Taiwan for the generous gift of rabbit anti-capsid serum against the dsRNA virus. The work in this paper was supported by Public Health Service Grants AI 43940 and AI 45429 from the National Institutes of Health (to JFA) and grants from Financiadora de Estudos e Projetos, Conselho Nacional de Desenvolvimento Cientifico e Tecnol{\'o}gico, Programa de N{\'u}cleos de Excelencia, Funda{\c c}{\~a}o Carlos Chagas Filho de Amparo {\`a} Pesquisa do Estado do Rio de Janeiro, PRONEX, and Associa{\c c}{\~a}o Universit{\'a}ria Santa {\'U}rsula (to MB). The assistance of Anna Lazzell of the Institutional Hybridoma Facility of the UTHSCSA is acknowledged. We are indebted to the staff of the Baltimore City Health Department Sexually Transmitted Disease Program (Baltimore). We thank Jeff Yuenger (Johns Hopkins School of Medicine, Baltimore) and Della Duncan (Baltimore City Health Department Sexually Transmitted Disease Program), for assistance and laboratory expertise. We are grateful to Dr. Jeanna Piper of the UTHSCSA for her assistance in obtaining the fresh clinical UT isolates used for comparative purposes.",
year = "2003",
month = jan,
doi = "10.1016/S0014-4894(03)00068-7",
language = "English (US)",
volume = "103",
pages = "44--50",
journal = "Experimental Parasitology",
issn = "0014-4894",
publisher = "Academic Press Inc.",
number = "1-2",
}