Trichomonas vaginalis: Evaluating capsid proteins of dsRNA viruses and the dsRNA virus within patients attending a sexually transmitted disease clinic

J. F. Alderete, K. A. Wendel, A. M. Rompalo, E. J. Erbelding, M. Benchimol, T. H. Chang

Research output: Contribution to journalArticle

Abstract

Some isolates of Trichomonas vaginalis, the number one, non-viral sexually transmitted disease agent, are infected with one or several distinct double stranded (ds)-RNA virus. Immune rabbit anti-capsid serum (IRS) reacted with the capsid protein of purified dsRNA virus of a subset of the virus-infected T. vaginalis isolates. A monoclonal antibody (mAb) that recognized the capsid protein reactive with the IRS was generated. Analysis of the virus capsid protein of virus-infected isolates by probing nitrocellulose blots with mAb revealed diversity among immunoreactivity and in the size of the reactive capsid protein. Despite difficulties in visualizing virus within parasites by cross-section electron microscopy, gold-conjugated mAb readily labeled the cytoplasm of virus-positive trichomonads. Finally and importantly, isolates infecting patients attending an STD clinic, 75% of which were virus-positive isolates, had capsid protein of the same size detected by mAb present in all dsRNA viruses.

Original languageEnglish (US)
Pages (from-to)44-50
Number of pages7
JournalExperimental Parasitology
Volume103
Issue number1-2
DOIs
StatePublished - Jan 2003

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Keywords

  • Patients
  • Sexually transmitted diseases
  • Trichomonas vaginalis
  • Urogenital
  • Vaginal
  • dsRNA virus

ASJC Scopus subject areas

  • Parasitology
  • Immunology
  • Infectious Diseases

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