By controlling conditions which affect the relative rates of the partial reactions in HMG-CoA synthesis, it has been possible to trap and partially characterize HMG (-SCoA)-S-enzyme, the postulated central intermediate in catalysis. At -25° in 25% ethanol, the breakdown of HMG (-SCoA)-S-enzyme to HMG-SCoA and HS-enzyme is slowed relative to its rate of formation from acetoacetyl-SCoA and acetyl-S-enzyme. Under these conditions label from acetoacetyl-S[3H]CoA or [14C]acetyl-S-enzyme can be trapped in the accumulated intermediate with [14C] activity appearing in the HMG moiety.
|Original language||English (US)|
|Number of pages||7|
|Journal||Biochemical and Biophysical Research Communications|
|State||Published - Mar 8 1976|
ASJC Scopus subject areas
- Molecular Biology
- Cell Biology