Trapping cDNAs encoding secreted proteins from the salivary glands of the malaria vector Anopheles gambiae

Bruno Arcà, Fabrizio Lombardo, Margareth De Lara Capurro, Alessandra Della Torre, George Dimopoulos, Anthony A. James, Mario Coluzzi

Research output: Contribution to journalArticle

Abstract

The signal sequence trap method was used to isolate cDNAs corresponding to proteins containing secretory leader peptides and whose genes are expressed specifically in the salivary glands of the malaria vector Anopheles gambiae. Fifteen unique cDNA fragments, ranging in size from 150 to 550 bp, were isolated and sequenced in a first round of immunoscreening in COS-7 cells. All but one of the cDNAs contained putative signal sequences at their 5' ends, suggesting that they were likely to encode secreted or transmembrane proteins. Expression analysis by reverse transcription-PCR showed that at least six cDNA fragments were expressed specifically in the salivary glands. Fragments showing a high degree of similarity to D7 and apyrase, two salivary gland-specific genes previously found in Aedes aegypti, were identified. Of interest, three different D7-related cDNAs that are likely to represent a new gene family were found in An. gambiae. Moreover, three salivary gland- specific cDNA fragments that do not show similarity to known proteins in the databases were identified, and the corresponding full length cDNAs were cloned and sequenced. RNA in situ hybridization to whole female salivary glands showed patterns of expression that overlap only in part those observed in the culicine mosquito A. aegypti.

Original languageEnglish (US)
Pages (from-to)1516-1521
Number of pages6
JournalProceedings of the National Academy of Sciences of the United States of America
Volume96
Issue number4
DOIs
StatePublished - Feb 16 1999
Externally publishedYes

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Salivary Proteins and Peptides
Anopheles gambiae
Malaria
Complementary DNA
Salivary Glands
Protein Sorting Signals
Apyrase
Genes
Protein Databases
Aedes
COS Cells
Culicidae
Reverse Transcription
In Situ Hybridization
Proteins
RNA
Polymerase Chain Reaction

ASJC Scopus subject areas

  • Genetics
  • General

Cite this

Trapping cDNAs encoding secreted proteins from the salivary glands of the malaria vector Anopheles gambiae. / Arcà, Bruno; Lombardo, Fabrizio; De Lara Capurro, Margareth; Della Torre, Alessandra; Dimopoulos, George; James, Anthony A.; Coluzzi, Mario.

In: Proceedings of the National Academy of Sciences of the United States of America, Vol. 96, No. 4, 16.02.1999, p. 1516-1521.

Research output: Contribution to journalArticle

Arcà, Bruno ; Lombardo, Fabrizio ; De Lara Capurro, Margareth ; Della Torre, Alessandra ; Dimopoulos, George ; James, Anthony A. ; Coluzzi, Mario. / Trapping cDNAs encoding secreted proteins from the salivary glands of the malaria vector Anopheles gambiae. In: Proceedings of the National Academy of Sciences of the United States of America. 1999 ; Vol. 96, No. 4. pp. 1516-1521.
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