Trapping and sequence analysis of 1138 putative exons from human chromosome 18

H. Chen, N. Wang, Y. Huo, P. Sklar, Dean F Mackinnon, James Bennett Potash, F. J. McMahon, S. E. Antonarakis, J Raymond Depaulo, Christopher A Ross, M. G. McInnis

Research output: Contribution to journalArticle

Abstract

In a search for novel genes on chromosome 18 (HC18), on which several regions have been linked to bipolar disorder, we applied exon trapping to HC18-specific cosmids. Among the 1138 exons trapped, 1052 of them have been mapped to HC18, and the remaining 86 have not been localized. No exons were localized to genomic regions other than HC18. BLAST database search revealed that 190 exons were identical to 98 Unigenes on HC18; 98 identical to additional 82 clusters of ESTs not present in the HC18 Unigene set; 39 homologous to genes from human and other species (e>10-3); and the remaining 811 exons had no significant homology to transcripts in public databases. The mapped exons were compared to the 867 annotated genes on HC18 in the Celera databases; 216 exons were identical to 104 Celera 'genes' and the remaining 836 exons were not found in the Celera databases. On average, there were two exons for a matched transcript (known genes and ESTs). Therefore, the 850 novel exons may represent hundreds of novel genes on chromosome 18.

Original languageEnglish (US)
Pages (from-to)619-623
Number of pages5
JournalMolecular Psychiatry
Volume8
Issue number6
DOIs
StatePublished - 2003

Fingerprint

Chromosomes, Human, Pair 18
Human Chromosomes
Sequence Analysis
Exons
Databases
Genes
Expressed Sequence Tags
Cosmids
Bipolar Disorder

Keywords

  • Bipolar disorder
  • BLAST
  • Exon trapping
  • Gene identification
  • Genome

ASJC Scopus subject areas

  • Molecular Biology
  • Psychiatry and Mental health

Cite this

Trapping and sequence analysis of 1138 putative exons from human chromosome 18. / Chen, H.; Wang, N.; Huo, Y.; Sklar, P.; Mackinnon, Dean F; Potash, James Bennett; McMahon, F. J.; Antonarakis, S. E.; Depaulo, J Raymond; Ross, Christopher A; McInnis, M. G.

In: Molecular Psychiatry, Vol. 8, No. 6, 2003, p. 619-623.

Research output: Contribution to journalArticle

Chen, H, Wang, N, Huo, Y, Sklar, P, Mackinnon, DF, Potash, JB, McMahon, FJ, Antonarakis, SE, Depaulo, JR, Ross, CA & McInnis, MG 2003, 'Trapping and sequence analysis of 1138 putative exons from human chromosome 18', Molecular Psychiatry, vol. 8, no. 6, pp. 619-623. https://doi.org/10.1038/sj.mp.4001288
Chen, H. ; Wang, N. ; Huo, Y. ; Sklar, P. ; Mackinnon, Dean F ; Potash, James Bennett ; McMahon, F. J. ; Antonarakis, S. E. ; Depaulo, J Raymond ; Ross, Christopher A ; McInnis, M. G. / Trapping and sequence analysis of 1138 putative exons from human chromosome 18. In: Molecular Psychiatry. 2003 ; Vol. 8, No. 6. pp. 619-623.
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