Transsynaptic transfer of retrogradely transported tetanus protein-peroxidase conjugates

Paul S. Fishman, Joseph M Savitt

Research output: Contribution to journalArticle

Abstract

Tetanus toxin and its atoxic binding fragment, C-fragment (CF), are the only known proteins which undergo extensive transfer from motoneurons to presynaptic terminals in the spinal cord. Intramuscular injection of CF conjugated to horseradish peroxidase (HRP) was used to elucidate the ultrastructural basis for this unique property. Motoneurons labeled by retrograde axonal transport contained enzymatic reaction product within cytoplasmic vesicles and were surrounded by presynaptic terminals with label in the synaptic cleft and synaptic vesicles. Membranous structures on both sides of the synapse remained labeled for several days after the injection. Nonsynaptic regions were virtually free of CF-HRP. Transport of CF may reflect the movement of the tetanus toxin receptor in a unique synaptic compartment.

Original languageEnglish (US)
Pages (from-to)197-203
Number of pages7
JournalExperimental Neurology
Volume106
Issue number2
DOIs
StatePublished - 1989
Externally publishedYes

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Presynaptic Terminals
Tetanus
Motor Neurons
Horseradish Peroxidase
Peroxidase
Cytoplasmic Vesicles
Tetanus Toxin
Axonal Transport
Synaptic Vesicles
Intramuscular Injections
Synapses
Spinal Cord
Proteins
Injections
tetanus toxin receptor

ASJC Scopus subject areas

  • Neuroscience(all)
  • Neurology

Cite this

Transsynaptic transfer of retrogradely transported tetanus protein-peroxidase conjugates. / Fishman, Paul S.; Savitt, Joseph M.

In: Experimental Neurology, Vol. 106, No. 2, 1989, p. 197-203.

Research output: Contribution to journalArticle

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