Transpupillary thermotherapy-induced modification of angiogenesis- and coagulation-related expression in the rat posterior fundus

Yoko N. Ito, Mitsuteru Ito, Hiroyasu Takita, Shin Yoneya, Gholam A. Peyman, Peter Gehlbach, Keisuke Mori

Research output: Contribution to journalArticle

Abstract

Purpose: To study gene expression changes in the rat retina and choroid following transpupillary thermotherapy (TTT) and to identify molecular mechanisms that may enhance treatment of choroidal neovascularization, complicating age-related macular degeneration. Methods: One fundus of Brown Norway rats was treated with an 810 nm diode laser while the contralateral fundus received no treatment. The mRNA was extracted and processed for cDNA microarray analysis. Genes with increased expression were validated by semiquantitative reverse transcription polymerase chain reaction (PCR) and quantitative real-time PCR (qRT-PCR). Results: Of the 14,815 cDNA elements on the array, 12 genes were up-regulated in TTT treated eyes. Upregulation of eight of these 12 genes could be verified by semiquantitative RT-PCR. The eight verified genes were EPCR, IL-1β, MCP-1, TSP-1, Fgl, Asns, MT-2, and NMDMC, which included 4 angiogenesis- and coagulation-related genes. Conclusions: This study demonstrates upregulation of angiogenesis- and coagulation-related genes following TTT. The response profile and its temporal relationships provide insight into the molecular mechanisms that lead to vascular occlusion and antiangiogenesis induced by TTT.

Original languageEnglish (US)
Pages (from-to)802-810
Number of pages9
JournalMolecular Vision
Volume12
StatePublished - Jul 20 2006

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Induced Hyperthermia
Genes
Up-Regulation
Thrombospondin 1
Polymerase Chain Reaction
Choroidal Neovascularization
Semiconductor Lasers
Choroid
Macular Degeneration
Microarray Analysis
Oligonucleotide Array Sequence Analysis
Interleukin-1
Reverse Transcription
Blood Vessels
Retina
Real-Time Polymerase Chain Reaction
Complementary DNA
Gene Expression
Messenger RNA
Therapeutics

ASJC Scopus subject areas

  • Ophthalmology

Cite this

Transpupillary thermotherapy-induced modification of angiogenesis- and coagulation-related expression in the rat posterior fundus. / Ito, Yoko N.; Ito, Mitsuteru; Takita, Hiroyasu; Yoneya, Shin; Peyman, Gholam A.; Gehlbach, Peter; Mori, Keisuke.

In: Molecular Vision, Vol. 12, 20.07.2006, p. 802-810.

Research output: Contribution to journalArticle

Ito, Yoko N. ; Ito, Mitsuteru ; Takita, Hiroyasu ; Yoneya, Shin ; Peyman, Gholam A. ; Gehlbach, Peter ; Mori, Keisuke. / Transpupillary thermotherapy-induced modification of angiogenesis- and coagulation-related expression in the rat posterior fundus. In: Molecular Vision. 2006 ; Vol. 12. pp. 802-810.
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