Transposon-mediated site-specific recombination: Identification of three binding sites for resolvase at the res sites of γδ and Tn 3

N. D.F. Grindley, M. R. Lauth, R. G. Wells, R. J. Wityk, J. J. Salvo, R. R. Reed

Research output: Contribution to journalArticle

Abstract

The tnpR gene product, resolvase, of the transposable element γδ mediates site-specific recombination between two copies of γδ directly repeated on the same replicon, and it negatively regulates transcription of the tnpA (transposase) gene and its own gene. The recombinational site, res, and the regulatory region both are located in the tnpA-tnpR intercistronic region. In studying the interaction of purified resolvase with DNA fragments derived from γδ and the related transposon, Tn 3, that span this region, we have demonstrated that three sites specifically bind resolvase. Site I overlaps the recombinational crossover point and both transcriptional promoters. Sites II and III cover most of the DNA between the crossover point and the translational start of the tnpR gene. These are the only binding sites we have detected in a region of about 400 base pairs centered on the crossover point. Studies of cointegrates that contain only part of the region that binds resolvase indicate that site I is not sufficient for efficient site-specific recombination and suggest that all three sites are probably required.

Original languageEnglish (US)
Pages (from-to)19-27
Number of pages9
JournalCell
Volume30
Issue number1
DOIs
StatePublished - Aug 1982

ASJC Scopus subject areas

  • Biochemistry, Genetics and Molecular Biology(all)

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