Transposition of cloned P elements into Drosophila germ line chromosomes

Allan C. Spradling, Gerald M. Rubin

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Abstract

Recombinant DNA carrying the 3-kilobase P transposable element was injected into Drosophila embryos of a strain that lacked such elements. Under optimum conditions, half of the surviving embryos showed evidence of P element-induced mutations in a fraction of their progeny. Direct analysis of the DNA of strains derived from such flies showed them to contain from one to five intact 3-kilobase P elements located at a wide variety of chromosomal sites. DNA sequences located outside the P element on the injected DNA were not transferred. Thus P elements can efficiently and selectively transpose from extrachromosomal DNA to the DNA of germ line chromosomes in Drosophila embryos. These observations provide the basis for efficient DNA-mediated gene transfer in Drosophila.

Original languageEnglish (US)
Pages (from-to)341-347
Number of pages7
JournalScience
Volume218
Issue number4570
StatePublished - 1982
Externally publishedYes

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    Spradling, A. C., & Rubin, G. M. (1982). Transposition of cloned P elements into Drosophila germ line chromosomes. Science, 218(4570), 341-347.