Transporter-independent processing of HIV-1 envelope protein for recognition by CD8+ T cells

Scott A. Hammond, Robert C Bollinger, Timothy W. Tobery, Robert F Siliciano

Research output: Contribution to journalArticle

Abstract

CD8+ cytolytic T lymphocytes (CTL) identify virally infected cells by recognizing processed viral antigen in association with class I major histocompatibility complex (MHC) molecules on infected cells1-4. Processing begins in the cytosol5-7 with the generation of peptides, possibly by a protease complex with MHC-encoded subunits, known as the proteasome8-11. Transport of the resulting cytosolic peptides into the endoplasmic reticulum for association with class I molecules is essential and probably involves a heterodimer of the MHC-encoded proteins, Tap-1 and Tap-212-17. The site of processing of viral envelope proteins is uncertain. These proteins are not present in the cytosol because of cotranslational translocation into the endoplasmic reticulum. We show here that the HIV-1 envelope (env) protein is processed in infected cells by a novel Tap-1/Tap-2-independent pathway that seems to be localized to the endoplasmic reticulum.

Original languageEnglish (US)
Pages (from-to)158-161
Number of pages4
JournalNature
Volume364
Issue number6433
StatePublished - Jul 8 1993

Fingerprint

Major Histocompatibility Complex
Endoplasmic Reticulum
HIV-1
T-Lymphocytes
Viral Envelope Proteins
Peptides
Proteins
Viral Antigens
Cytosol
Peptide Hydrolases

ASJC Scopus subject areas

  • General

Cite this

Transporter-independent processing of HIV-1 envelope protein for recognition by CD8+ T cells. / Hammond, Scott A.; Bollinger, Robert C; Tobery, Timothy W.; Siliciano, Robert F.

In: Nature, Vol. 364, No. 6433, 08.07.1993, p. 158-161.

Research output: Contribution to journalArticle

@article{3a0843b11f0c4e64bac33111366072c2,
title = "Transporter-independent processing of HIV-1 envelope protein for recognition by CD8+ T cells",
abstract = "CD8+ cytolytic T lymphocytes (CTL) identify virally infected cells by recognizing processed viral antigen in association with class I major histocompatibility complex (MHC) molecules on infected cells1-4. Processing begins in the cytosol5-7 with the generation of peptides, possibly by a protease complex with MHC-encoded subunits, known as the proteasome8-11. Transport of the resulting cytosolic peptides into the endoplasmic reticulum for association with class I molecules is essential and probably involves a heterodimer of the MHC-encoded proteins, Tap-1 and Tap-212-17. The site of processing of viral envelope proteins is uncertain. These proteins are not present in the cytosol because of cotranslational translocation into the endoplasmic reticulum. We show here that the HIV-1 envelope (env) protein is processed in infected cells by a novel Tap-1/Tap-2-independent pathway that seems to be localized to the endoplasmic reticulum.",
author = "Hammond, {Scott A.} and Bollinger, {Robert C} and Tobery, {Timothy W.} and Siliciano, {Robert F}",
year = "1993",
month = "7",
day = "8",
language = "English (US)",
volume = "364",
pages = "158--161",
journal = "Nature",
issn = "0028-0836",
publisher = "Nature Publishing Group",
number = "6433",

}

TY - JOUR

T1 - Transporter-independent processing of HIV-1 envelope protein for recognition by CD8+ T cells

AU - Hammond, Scott A.

AU - Bollinger, Robert C

AU - Tobery, Timothy W.

AU - Siliciano, Robert F

PY - 1993/7/8

Y1 - 1993/7/8

N2 - CD8+ cytolytic T lymphocytes (CTL) identify virally infected cells by recognizing processed viral antigen in association with class I major histocompatibility complex (MHC) molecules on infected cells1-4. Processing begins in the cytosol5-7 with the generation of peptides, possibly by a protease complex with MHC-encoded subunits, known as the proteasome8-11. Transport of the resulting cytosolic peptides into the endoplasmic reticulum for association with class I molecules is essential and probably involves a heterodimer of the MHC-encoded proteins, Tap-1 and Tap-212-17. The site of processing of viral envelope proteins is uncertain. These proteins are not present in the cytosol because of cotranslational translocation into the endoplasmic reticulum. We show here that the HIV-1 envelope (env) protein is processed in infected cells by a novel Tap-1/Tap-2-independent pathway that seems to be localized to the endoplasmic reticulum.

AB - CD8+ cytolytic T lymphocytes (CTL) identify virally infected cells by recognizing processed viral antigen in association with class I major histocompatibility complex (MHC) molecules on infected cells1-4. Processing begins in the cytosol5-7 with the generation of peptides, possibly by a protease complex with MHC-encoded subunits, known as the proteasome8-11. Transport of the resulting cytosolic peptides into the endoplasmic reticulum for association with class I molecules is essential and probably involves a heterodimer of the MHC-encoded proteins, Tap-1 and Tap-212-17. The site of processing of viral envelope proteins is uncertain. These proteins are not present in the cytosol because of cotranslational translocation into the endoplasmic reticulum. We show here that the HIV-1 envelope (env) protein is processed in infected cells by a novel Tap-1/Tap-2-independent pathway that seems to be localized to the endoplasmic reticulum.

UR - http://www.scopus.com/inward/record.url?scp=0027237066&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=0027237066&partnerID=8YFLogxK

M3 - Article

VL - 364

SP - 158

EP - 161

JO - Nature

JF - Nature

SN - 0028-0836

IS - 6433

ER -