Transport of polymeric nanoparticle gene carriers in gastric mucus

Michelle Dawson, Eric Krauland, Denis Wirtz, Justin S Hanes

Research output: Contribution to journalArticle

Abstract

Nanoparticle transport through mucosal barriers is often restricted owing to mucoadhesion and the highly viscoelastic nature of mucus gels, which may limit efficient drug and gene delivery. We formulated sub-200 nm particulates from poly(D,L-lactic-coglycolic) acid (PLGA) and the cationic surfactant dimethyldioctadecylammonium bromide (DDAB). Subsequently, anionic DNA was condensed to the surface to obtain gene carriers with transfection rates 50-fold higher than those of naked DNA in vitro. Using the method of multiple particle tracking (MPT), we measured the transport rates of dozens of individual PLGA-DDAB/DNA nanoparticles in real time in reconstituted pig gastric mucus (PGM) that possessed physiologically relevant rheological properties. The average transport rate of PLGA-DDAB/DNA nanoparticles was 10-fold higher than those of similar size polystyrene nanoparticles. Improved transport rates, stability in mucus, and ability to transfect cells make PLGA-DDAB/DNA nanoparticles candidates for mucosal DNA vaccines and gene therapy.

Original languageEnglish (US)
Pages (from-to)851-857
Number of pages7
JournalBiotechnology Progress
Volume20
Issue number3
DOIs
StatePublished - May 2004

Fingerprint

nanoparticles
Mucus
mucus
Nanoparticles
Stomach
stomach
bromides
DNA
Genes
genes
Genetic Therapy
Active Immunotherapy
DNA Vaccines
gene therapy
Polystyrenes
polystyrenes
recombinant vaccines
transfection
rheological properties
gene transfer

ASJC Scopus subject areas

  • Food Science
  • Biotechnology
  • Microbiology

Cite this

Transport of polymeric nanoparticle gene carriers in gastric mucus. / Dawson, Michelle; Krauland, Eric; Wirtz, Denis; Hanes, Justin S.

In: Biotechnology Progress, Vol. 20, No. 3, 05.2004, p. 851-857.

Research output: Contribution to journalArticle

@article{797ff65384c4493886ae88c1df905c70,
title = "Transport of polymeric nanoparticle gene carriers in gastric mucus",
abstract = "Nanoparticle transport through mucosal barriers is often restricted owing to mucoadhesion and the highly viscoelastic nature of mucus gels, which may limit efficient drug and gene delivery. We formulated sub-200 nm particulates from poly(D,L-lactic-coglycolic) acid (PLGA) and the cationic surfactant dimethyldioctadecylammonium bromide (DDAB). Subsequently, anionic DNA was condensed to the surface to obtain gene carriers with transfection rates 50-fold higher than those of naked DNA in vitro. Using the method of multiple particle tracking (MPT), we measured the transport rates of dozens of individual PLGA-DDAB/DNA nanoparticles in real time in reconstituted pig gastric mucus (PGM) that possessed physiologically relevant rheological properties. The average transport rate of PLGA-DDAB/DNA nanoparticles was 10-fold higher than those of similar size polystyrene nanoparticles. Improved transport rates, stability in mucus, and ability to transfect cells make PLGA-DDAB/DNA nanoparticles candidates for mucosal DNA vaccines and gene therapy.",
author = "Michelle Dawson and Eric Krauland and Denis Wirtz and Hanes, {Justin S}",
year = "2004",
month = "5",
doi = "10.1021/bp0342553",
language = "English (US)",
volume = "20",
pages = "851--857",
journal = "Biotechnology Progress",
issn = "8756-7938",
publisher = "John Wiley and Sons Ltd",
number = "3",

}

TY - JOUR

T1 - Transport of polymeric nanoparticle gene carriers in gastric mucus

AU - Dawson, Michelle

AU - Krauland, Eric

AU - Wirtz, Denis

AU - Hanes, Justin S

PY - 2004/5

Y1 - 2004/5

N2 - Nanoparticle transport through mucosal barriers is often restricted owing to mucoadhesion and the highly viscoelastic nature of mucus gels, which may limit efficient drug and gene delivery. We formulated sub-200 nm particulates from poly(D,L-lactic-coglycolic) acid (PLGA) and the cationic surfactant dimethyldioctadecylammonium bromide (DDAB). Subsequently, anionic DNA was condensed to the surface to obtain gene carriers with transfection rates 50-fold higher than those of naked DNA in vitro. Using the method of multiple particle tracking (MPT), we measured the transport rates of dozens of individual PLGA-DDAB/DNA nanoparticles in real time in reconstituted pig gastric mucus (PGM) that possessed physiologically relevant rheological properties. The average transport rate of PLGA-DDAB/DNA nanoparticles was 10-fold higher than those of similar size polystyrene nanoparticles. Improved transport rates, stability in mucus, and ability to transfect cells make PLGA-DDAB/DNA nanoparticles candidates for mucosal DNA vaccines and gene therapy.

AB - Nanoparticle transport through mucosal barriers is often restricted owing to mucoadhesion and the highly viscoelastic nature of mucus gels, which may limit efficient drug and gene delivery. We formulated sub-200 nm particulates from poly(D,L-lactic-coglycolic) acid (PLGA) and the cationic surfactant dimethyldioctadecylammonium bromide (DDAB). Subsequently, anionic DNA was condensed to the surface to obtain gene carriers with transfection rates 50-fold higher than those of naked DNA in vitro. Using the method of multiple particle tracking (MPT), we measured the transport rates of dozens of individual PLGA-DDAB/DNA nanoparticles in real time in reconstituted pig gastric mucus (PGM) that possessed physiologically relevant rheological properties. The average transport rate of PLGA-DDAB/DNA nanoparticles was 10-fold higher than those of similar size polystyrene nanoparticles. Improved transport rates, stability in mucus, and ability to transfect cells make PLGA-DDAB/DNA nanoparticles candidates for mucosal DNA vaccines and gene therapy.

UR - http://www.scopus.com/inward/record.url?scp=2942735394&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=2942735394&partnerID=8YFLogxK

U2 - 10.1021/bp0342553

DO - 10.1021/bp0342553

M3 - Article

VL - 20

SP - 851

EP - 857

JO - Biotechnology Progress

JF - Biotechnology Progress

SN - 8756-7938

IS - 3

ER -