Transport of polymeric nanoparticle gene carriers in gastric mucus

Michelle Dawson, Eric Krauland, Denis Wirtz, Justin Hanes

Research output: Contribution to journalArticle

Abstract

Nanoparticle transport through mucosal barriers is often restricted owing to mucoadhesion and the highly viscoelastic nature of mucus gels, which may limit efficient drug and gene delivery. We formulated sub-200 nm particulates from poly(D,L-lactic-coglycolic) acid (PLGA) and the cationic surfactant dimethyldioctadecylammonium bromide (DDAB). Subsequently, anionic DNA was condensed to the surface to obtain gene carriers with transfection rates 50-fold higher than those of naked DNA in vitro. Using the method of multiple particle tracking (MPT), we measured the transport rates of dozens of individual PLGA-DDAB/DNA nanoparticles in real time in reconstituted pig gastric mucus (PGM) that possessed physiologically relevant rheological properties. The average transport rate of PLGA-DDAB/DNA nanoparticles was 10-fold higher than those of similar size polystyrene nanoparticles. Improved transport rates, stability in mucus, and ability to transfect cells make PLGA-DDAB/DNA nanoparticles candidates for mucosal DNA vaccines and gene therapy.

Original languageEnglish (US)
Pages (from-to)851-857
Number of pages7
JournalBiotechnology Progress
Volume20
Issue number3
DOIs
StatePublished - May 1 2004

ASJC Scopus subject areas

  • Biotechnology

Fingerprint Dive into the research topics of 'Transport of polymeric nanoparticle gene carriers in gastric mucus'. Together they form a unique fingerprint.

  • Cite this