Transport of gallocatechin gallate and catechin gallate in high-temperature-processed green tea extract from gastrointestinal tract to brain by an in vitro bio-mimic model system coupled with sequential cell cultures

Kang Hyun Jeong, Si Young Cho, Yong Deog Hong, Jin Oh Chung, Kwang Sik Kim, Soon Mi Shim

Research output: Contribution to journalArticle

Abstract

The aim of this study was to evaluate the transport of gallocatechin gallate (GCG), catechin gallate (CG) and their metabolites in high-temperature-processed green tea extract (HTP_GTE) from the gastrointestinal tract to blood-brain barrier (BBB) with an in vitro bio-mimic model system with sequential cell cultures including Caco-2, HepG2 and HBMECs. Transports from the GI to BBB, the final concentrations of GCC and CG were 18.95 ± 3.24 μM and 5.11 ± 0.83 μM, respectively. Metabolites detected in BBB after systemic circulation were identified as glucuronide or sulfate conjugated form. Results suggest that GCG, CG and their metabolites in HTP_GTE are capable of reaching the brain by oral intake of HTP_GTE, implying that HTP_GTE could be utilized as a natural functional material for the prevention of degenerative brain diseases.

Original languageEnglish (US)
Pages (from-to)83-90
Number of pages8
JournalJournal of Functional Foods
Volume47
DOIs
StatePublished - Aug 1 2018

Fingerprint

epigallocatechin
green tea
Tea
catechin
gastrointestinal system
blood-brain barrier
Gastrointestinal Tract
cell culture
Cell Culture Techniques
Blood-Brain Barrier
brain
Temperature
extracts
Brain
metabolites
temperature
central nervous system diseases
Glucuronides
Brain Diseases
Sulfates

Keywords

  • CG
  • GCG
  • In vitro bio-mimic model
  • Metabolite

ASJC Scopus subject areas

  • Food Science
  • Medicine (miscellaneous)
  • Nutrition and Dietetics

Cite this

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title = "Transport of gallocatechin gallate and catechin gallate in high-temperature-processed green tea extract from gastrointestinal tract to brain by an in vitro bio-mimic model system coupled with sequential cell cultures",
abstract = "The aim of this study was to evaluate the transport of gallocatechin gallate (GCG), catechin gallate (CG) and their metabolites in high-temperature-processed green tea extract (HTP_GTE) from the gastrointestinal tract to blood-brain barrier (BBB) with an in vitro bio-mimic model system with sequential cell cultures including Caco-2, HepG2 and HBMECs. Transports from the GI to BBB, the final concentrations of GCC and CG were 18.95 ± 3.24 μM and 5.11 ± 0.83 μM, respectively. Metabolites detected in BBB after systemic circulation were identified as glucuronide or sulfate conjugated form. Results suggest that GCG, CG and their metabolites in HTP_GTE are capable of reaching the brain by oral intake of HTP_GTE, implying that HTP_GTE could be utilized as a natural functional material for the prevention of degenerative brain diseases.",
keywords = "CG, GCG, In vitro bio-mimic model, Metabolite",
author = "Jeong, {Kang Hyun} and Cho, {Si Young} and Hong, {Yong Deog} and Chung, {Jin Oh} and Kim, {Kwang Sik} and Shim, {Soon Mi}",
year = "2018",
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T1 - Transport of gallocatechin gallate and catechin gallate in high-temperature-processed green tea extract from gastrointestinal tract to brain by an in vitro bio-mimic model system coupled with sequential cell cultures

AU - Jeong, Kang Hyun

AU - Cho, Si Young

AU - Hong, Yong Deog

AU - Chung, Jin Oh

AU - Kim, Kwang Sik

AU - Shim, Soon Mi

PY - 2018/8/1

Y1 - 2018/8/1

N2 - The aim of this study was to evaluate the transport of gallocatechin gallate (GCG), catechin gallate (CG) and their metabolites in high-temperature-processed green tea extract (HTP_GTE) from the gastrointestinal tract to blood-brain barrier (BBB) with an in vitro bio-mimic model system with sequential cell cultures including Caco-2, HepG2 and HBMECs. Transports from the GI to BBB, the final concentrations of GCC and CG were 18.95 ± 3.24 μM and 5.11 ± 0.83 μM, respectively. Metabolites detected in BBB after systemic circulation were identified as glucuronide or sulfate conjugated form. Results suggest that GCG, CG and their metabolites in HTP_GTE are capable of reaching the brain by oral intake of HTP_GTE, implying that HTP_GTE could be utilized as a natural functional material for the prevention of degenerative brain diseases.

AB - The aim of this study was to evaluate the transport of gallocatechin gallate (GCG), catechin gallate (CG) and their metabolites in high-temperature-processed green tea extract (HTP_GTE) from the gastrointestinal tract to blood-brain barrier (BBB) with an in vitro bio-mimic model system with sequential cell cultures including Caco-2, HepG2 and HBMECs. Transports from the GI to BBB, the final concentrations of GCC and CG were 18.95 ± 3.24 μM and 5.11 ± 0.83 μM, respectively. Metabolites detected in BBB after systemic circulation were identified as glucuronide or sulfate conjugated form. Results suggest that GCG, CG and their metabolites in HTP_GTE are capable of reaching the brain by oral intake of HTP_GTE, implying that HTP_GTE could be utilized as a natural functional material for the prevention of degenerative brain diseases.

KW - CG

KW - GCG

KW - In vitro bio-mimic model

KW - Metabolite

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