Transport of gallocatechin gallate and catechin gallate in high-temperature-processed green tea extract from gastrointestinal tract to brain by an in vitro bio-mimic model system coupled with sequential cell cultures

Kang Hyun Jeong, Si Young Cho, Yong Deog Hong, Jin Oh Chung, Kwang Sik Kim, Soon Mi Shim

Research output: Contribution to journalArticlepeer-review

Abstract

The aim of this study was to evaluate the transport of gallocatechin gallate (GCG), catechin gallate (CG) and their metabolites in high-temperature-processed green tea extract (HTP_GTE) from the gastrointestinal tract to blood-brain barrier (BBB) with an in vitro bio-mimic model system with sequential cell cultures including Caco-2, HepG2 and HBMECs. Transports from the GI to BBB, the final concentrations of GCC and CG were 18.95 ± 3.24 μM and 5.11 ± 0.83 μM, respectively. Metabolites detected in BBB after systemic circulation were identified as glucuronide or sulfate conjugated form. Results suggest that GCG, CG and their metabolites in HTP_GTE are capable of reaching the brain by oral intake of HTP_GTE, implying that HTP_GTE could be utilized as a natural functional material for the prevention of degenerative brain diseases.

Original languageEnglish (US)
Pages (from-to)83-90
Number of pages8
JournalJournal of Functional Foods
Volume47
DOIs
StatePublished - Aug 2018

Keywords

  • CG
  • GCG
  • In vitro bio-mimic model
  • Metabolite

ASJC Scopus subject areas

  • Food Science
  • Medicine (miscellaneous)
  • Nutrition and Dietetics

Fingerprint Dive into the research topics of 'Transport of gallocatechin gallate and catechin gallate in high-temperature-processed green tea extract from gastrointestinal tract to brain by an in vitro bio-mimic model system coupled with sequential cell cultures'. Together they form a unique fingerprint.

Cite this