Transport and phosphorylation of hexoses in normal and Rous sarcoma virus-transformed chick embryo fibroblasts

M. Singh, V. N. Singh, Thomas August, B. L. Horecker

Research output: Contribution to journalArticle

Abstract

Effects of transformation by Rous sarcoma virus on sugar uptake and activity and the subcellular distribution of hexokinase isozymes in chick embryo fibroblasts were examined. Transformation caused a several-fold increase in the maximum velocity for uptake of 2-deoxyglucose without a significant change in Km. Cytochalasin B (CB), was used to differentiate between the effects of transformation on facilitated diffusion and the nonsaturable (CB-insensitive) mode. Transformation was found to stimulate 2-deoxyglucose transport by both mechanisms, but the increase in transport by the CB-insensitive mode was greater. Transformation enhances the activity of hexokinase, the enhancement being confined to the particulate fraction of the enzyme. Heat-inactivation and electrophoretic mobility studies showed that although hexokinase Type I is the major form in both normal and transformed fibroblasts, there is a significant increase in the proportion of the Type II isozyme in the transformed cells.

Original languageEnglish (US)
Pages (from-to)285-292
Number of pages8
JournalJournal of Cellular Physiology
Volume97
Issue number3 I
StatePublished - 1978

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Rous sarcoma virus
Phosphorylation
Cytochalasin B
Hexokinase
Hexoses
Chick Embryo
Fibroblasts
Viruses
Deoxyglucose
Isoenzymes
Facilitated Diffusion
Electrophoretic mobility
Sugars
Hot Temperature
Enzymes

ASJC Scopus subject areas

  • Cell Biology
  • Clinical Biochemistry
  • Physiology

Cite this

Transport and phosphorylation of hexoses in normal and Rous sarcoma virus-transformed chick embryo fibroblasts. / Singh, M.; Singh, V. N.; August, Thomas; Horecker, B. L.

In: Journal of Cellular Physiology, Vol. 97, No. 3 I, 1978, p. 285-292.

Research output: Contribution to journalArticle

Singh, M. ; Singh, V. N. ; August, Thomas ; Horecker, B. L. / Transport and phosphorylation of hexoses in normal and Rous sarcoma virus-transformed chick embryo fibroblasts. In: Journal of Cellular Physiology. 1978 ; Vol. 97, No. 3 I. pp. 285-292.
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