Abstract
Dissociated photoreceptor cells from a transgenic strain of mice, containing a bovine promoter lac Z gene construct, were transplanted to the dystrophic retinas of a strain of mice (C3H, rd/rd) without obvious photoreceptors. The transgenic photoreceptor cells expressed β-galactosidase and were distinguishable from the host retinal cells by light and electron microscopy after the 5-bromo-4-chloro-3-indolyl-β-D-galactopyranoside histochemical reaction. These results showed that transplanted transgenic photoreceptor cells survived at least 1 month in the host retina and had a primitive outer segment and a well-developed synaptic terminal.
Original language | English (US) |
---|---|
Pages (from-to) | 3167-3174 |
Number of pages | 8 |
Journal | Investigative Ophthalmology and Visual Science |
Volume | 32 |
Issue number | 13 |
State | Published - Dec 1 1991 |
Externally published | Yes |
Keywords
- C3H mice
- lac Z reporter gene
- photoreceptors
- transgenic mice
- transplantation
ASJC Scopus subject areas
- Ophthalmology
- Sensory Systems
- Cellular and Molecular Neuroscience