Transplanted photoreceptors identified in dystrophic mouse retina by a transgenic reporter gene

P. Gouras, J. Du, H. Kjeldbye, R. Kwun, R. Lopez, D. J. Zack

Research output: Contribution to journalArticlepeer-review

Abstract

Dissociated photoreceptor cells from a transgenic strain of mice, containing a bovine promoter lac Z gene construct, were transplanted to the dystrophic retinas of a strain of mice (C3H, rd/rd) without obvious photoreceptors. The transgenic photoreceptor cells expressed β-galactosidase and were distinguishable from the host retinal cells by light and electron microscopy after the 5-bromo-4-chloro-3-indolyl-β-D-galactopyranoside histochemical reaction. These results showed that transplanted transgenic photoreceptor cells survived at least 1 month in the host retina and had a primitive outer segment and a well-developed synaptic terminal.

Original languageEnglish (US)
Pages (from-to)3167-3174
Number of pages8
JournalInvestigative Ophthalmology and Visual Science
Volume32
Issue number13
StatePublished - Dec 1 1991
Externally publishedYes

Keywords

  • C3H mice
  • lac Z reporter gene
  • photoreceptors
  • transgenic mice
  • transplantation

ASJC Scopus subject areas

  • Ophthalmology
  • Sensory Systems
  • Cellular and Molecular Neuroscience

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