Transplantation of pulmonary valve using a mouse model of heterotopic heart transplantation

Yong Ung Lee, Tai Yi, Iyore James, Shuhei Tara, Alexander J. Stuber, Kejal V. Shah, Avione Y. Lee, Tadahisa Sugiura, Narutoshi Hibino, Toshiharu Shinoka, Christopher K. Breuer

Research output: Contribution to journalArticle

Abstract

Tissue engineered heart valves, especially decellularized valves, are starting to gain momentum in clinical use of reconstructive surgery with mixed results. However, the cellular and molecular mechanisms of the neotissue development, valve thickening, and stenosis development are not researched extensively. To answer the above questions, we developed a murine heterotopic heart valve transplantation model. A heart valve was harvested from a valve donor mouse and transplanted to a heart donor mouse. The heart with a new valve was transplanted heterotopically to a recipient mouse. The transplanted heart showed its own heartbeat, independent of the recipient's heartbeat. The blood flow was quantified using a high frequency ultrasound system with a pulsed wave Doppler. The flow through the implanted pulmonary valve showed forward flow with minimal regurgitation and the peak flow was close to 100 mm/sec. This murine model of heart valve transplantation is highly versatile, so it can be modified and adapted to provide different hemodynamic environments and/or can be used with various transgenic mice to study neotissue development in a tissue engineered heart valve.

Original languageEnglish (US)
Article numbere51695
JournalJournal of Visualized Experiments
Issue number89
DOIs
StatePublished - Jul 23 2014
Externally publishedYes

Fingerprint

Heterotopic Transplantation
Pulmonary Valve
Heart Valves
Heart Transplantation
Transplantation
Reconstructive Surgical Procedures
Transgenic Mice
Tissue
Pathologic Constriction
Hemodynamics
Surgery
Momentum
Blood
Ultrasonics

Keywords

  • Congenital heart defect
  • Decellularized heart valve
  • Heterotopic heart transplantation
  • Issue 89
  • Medicine
  • Pulmonary valve
  • Tissue engineering
  • Transgenic mouse model

ASJC Scopus subject areas

  • Biochemistry, Genetics and Molecular Biology(all)
  • Chemical Engineering(all)
  • Immunology and Microbiology(all)
  • Neuroscience(all)

Cite this

Lee, Y. U., Yi, T., James, I., Tara, S., Stuber, A. J., Shah, K. V., ... Breuer, C. K. (2014). Transplantation of pulmonary valve using a mouse model of heterotopic heart transplantation. Journal of Visualized Experiments, (89), [e51695]. https://doi.org/10.3791/51695

Transplantation of pulmonary valve using a mouse model of heterotopic heart transplantation. / Lee, Yong Ung; Yi, Tai; James, Iyore; Tara, Shuhei; Stuber, Alexander J.; Shah, Kejal V.; Lee, Avione Y.; Sugiura, Tadahisa; Hibino, Narutoshi; Shinoka, Toshiharu; Breuer, Christopher K.

In: Journal of Visualized Experiments, No. 89, e51695, 23.07.2014.

Research output: Contribution to journalArticle

Lee, YU, Yi, T, James, I, Tara, S, Stuber, AJ, Shah, KV, Lee, AY, Sugiura, T, Hibino, N, Shinoka, T & Breuer, CK 2014, 'Transplantation of pulmonary valve using a mouse model of heterotopic heart transplantation', Journal of Visualized Experiments, no. 89, e51695. https://doi.org/10.3791/51695
Lee, Yong Ung ; Yi, Tai ; James, Iyore ; Tara, Shuhei ; Stuber, Alexander J. ; Shah, Kejal V. ; Lee, Avione Y. ; Sugiura, Tadahisa ; Hibino, Narutoshi ; Shinoka, Toshiharu ; Breuer, Christopher K. / Transplantation of pulmonary valve using a mouse model of heterotopic heart transplantation. In: Journal of Visualized Experiments. 2014 ; No. 89.
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