Transplantation of neural stem cells derived from human cord blood to the brain of adult and neonatal rats

Anna Jablonska, Hanna Kozlowska, Inga Markiewicz, Krystyna Domanska-Janik, Barbara Lukomska

Research output: Contribution to journalArticlepeer-review

Abstract

Searching for a reliable source of alternative neural stem cells for experimental treatment of neurological disorders we have established neural stem cell line derived from human umbilical cord blood (HUCB-NSC) (Buzanska et al. 2006). These cells have been shown to differentiate along neuronal and glial lineages in the promoting in vitro conditions. In the current study we transplanted HUCB-NSC into rat brain to determine whether the neural progenitors would be able to survive, migrate and eventually adopt neural phenotypes after exposure to central nervous system (CNS) microenvironment. Our experiments revealed that HUCB-NSC grafting into the brain of adult rats limited their survival up-to two weeks probably due to their elimination by severe immunological host reaction evoked by xenotransplantation. HUCB-NSC graft in neonates survived longer time in rat brain, migrated, proliferated and differentiated into neuronal cells however their presence in the host tissue did not exceed more than five weeks after transplantation.

Original languageEnglish (US)
Pages (from-to)337-350
Number of pages14
JournalActa Neurobiologiae Experimentalis
Volume70
Issue number4
StatePublished - Dec 1 2010
Externally publishedYes

Keywords

  • Cell transplantation
  • Cord blood
  • Immune response
  • Neural stem cells
  • Rat brain

ASJC Scopus subject areas

  • Neuroscience(all)

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