Transplantation of a novel human cord blood-derived neural-like stem cell line in a rat model of cortical infarct

Hanna Kozłowska, Jan Jabłonka, Miroslaw Janowski, Marcin Jurga, Malgorzata Kossut, Krystyna Domańska-Janik

Research output: Contribution to journalArticle

Abstract

Umbilical cord blood can be a rich source of stem/progenitor cells, not only for hematopoetic but also for other tissue-specific lineages. Recently, we have developed a novel, self-renewed neural-like stem cell line named HUCB-NSC from human cord blood. To test if HUCB-NSCs can supply brain in need of regeneration, we injected these cells into immunosuppressed intact rat forebrain and to animals suffering from a photothrombotic cortical lesion at 48 h after injury. The survival, migration, and differentiation of the transplanted HUCB-NSCs were measured at 7 and 30 days post-transplantation by immunohistochemical methods. Results show survival and extensive migration of transplanted neural-like progenitors into damaged brain cortex during the first week of post-stroke recovery. The donor cells accumulated mainly in peri-infarct area and then differentiated showing a strong co-expression of neuronal (NF-200) but only moderate of astrocytic (GFAP) cell markers. However, the paucity of HUCB-NSCs detected within post-ischemic rat brain at the end of a 1 month period, as well as acute rejection of grafted cells by intact, yet cyclosporin A (CsA) immunosuppressed, rat brain tissue, suggests development of a severe adverse host reaction to the presence of alien donor cells and an urgent need for further study of the immunological response evoked by xenotransplantations of human cord blood-derived cells in animal experimental models.

Original languageEnglish (US)
Pages (from-to)481-488
Number of pages8
JournalStem Cells and Development
Volume16
Issue number3
DOIs
StatePublished - Jun 2007
Externally publishedYes

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Neural Stem Cells
Fetal Blood
Transplantation
Cell Line
Brain
Stem Cells
Tissue Donors
Heterologous Transplantation
Survival
Prosencephalon
Cyclosporine
Regeneration
Blood Cells
Animal Models
Stroke
Wounds and Injuries

ASJC Scopus subject areas

  • Hematology

Cite this

Kozłowska, H., Jabłonka, J., Janowski, M., Jurga, M., Kossut, M., & Domańska-Janik, K. (2007). Transplantation of a novel human cord blood-derived neural-like stem cell line in a rat model of cortical infarct. Stem Cells and Development, 16(3), 481-488. https://doi.org/10.1089/scd.2007.9993

Transplantation of a novel human cord blood-derived neural-like stem cell line in a rat model of cortical infarct. / Kozłowska, Hanna; Jabłonka, Jan; Janowski, Miroslaw; Jurga, Marcin; Kossut, Malgorzata; Domańska-Janik, Krystyna.

In: Stem Cells and Development, Vol. 16, No. 3, 06.2007, p. 481-488.

Research output: Contribution to journalArticle

Kozłowska, H, Jabłonka, J, Janowski, M, Jurga, M, Kossut, M & Domańska-Janik, K 2007, 'Transplantation of a novel human cord blood-derived neural-like stem cell line in a rat model of cortical infarct', Stem Cells and Development, vol. 16, no. 3, pp. 481-488. https://doi.org/10.1089/scd.2007.9993
Kozłowska, Hanna ; Jabłonka, Jan ; Janowski, Miroslaw ; Jurga, Marcin ; Kossut, Malgorzata ; Domańska-Janik, Krystyna. / Transplantation of a novel human cord blood-derived neural-like stem cell line in a rat model of cortical infarct. In: Stem Cells and Development. 2007 ; Vol. 16, No. 3. pp. 481-488.
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