Transplantation in miniature swine: II. In vitro parameters of histocompatibility in MSLA homozygous minipigs

George S. Leight, David H. Sachs, Steven A. Rosenberg

Research output: Contribution to journalArticle

Abstract

A selective breeding program based on serological techniques was used to establish three herds of miniature swine, each homozygous for a different allele at the major histocompatibility complex, termed MSLA. By selective immunization of these swine, high-titered antisera detecting the products of individual MSLA alleles were raised and used to confirm the genotype of offspring in these herds. Techniques for performing one-way mixed leukocyte cultures in these pigs were developed and demonstrated the presence of a strong mixed leukocyte culture locus (or loci) closely linked to the serologically defined MSLA locus. Cell-mediated cytotoxicity assays based on 51Cr release were developed to monitor the course of cellular immunity following allotrans-plantation and exhibited specificity identical to that predicted by the serological typing.

Original languageEnglish (US)
Pages (from-to)271-276
Number of pages6
JournalTransplantation
Volume23
Issue number3
DOIs
StatePublished - Mar 1977

ASJC Scopus subject areas

  • Transplantation

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