Transnuclear CD8 T cells specific for the immunodominant epitope Gra6 lower acute-phase Toxoplasma gondii burden

Anna Sanecka, Nagisa Yoshida, Stephanie K. Dougan, John Jackson, Nilabh Shastri, Hidde Ploegh, Nicolas Blanchard, Eva Maria Frickel

Research output: Contribution to journalArticle

Abstract

We generated a CD8 T-cell receptor (TCR) transnuclear (TN) mouse specific to the Ld-restricted immunodominant epitope of GRA6 from Toxoplasma gondii as a source of cells to facilitate further investigation into the CD8 T-cell-mediated response against this pathogen. The TN T cells bound Ld-Gra6 tetramer and proliferated upon unspecific and peptide-specific stimulation. The TCR beta sequence of the Gra6-specific TN CD8 T cells is identical in its V- and J-region to the TCR-β harboured by a hybridoma line generated in response to Gra6 peptide. Adoptively transferred Gra6 TN CD8 T cells proliferated upon Toxoplasma infection in vivo and exhibited an activated phenotype similar to host CD8 T cells specific to Gra6. The brain of Toxoplasma-infected mice carried Gra6 TN cells already at day 8 post-infection. Both Gra6 TN mice as well as adoptively transferred Gra6 TN cells were able to significantly reduce the parasite burden in the acute phase of Toxoplasma infection. Overall, the Gra6 TN mouse represents a functional tool to study the protective and immunodominant specific CD8 T-cell response to Toxoplasma in both the acute and the chronic phases of infection.

Original languageEnglish (US)
Pages (from-to)270-279
Number of pages10
JournalImmunology
Volume149
Issue number3
DOIs
StatePublished - Nov 1 2016

Keywords

  • T cells
  • antigens/peptides/epitopes
  • parasitic protozoan

ASJC Scopus subject areas

  • Immunology and Allergy
  • Immunology

Fingerprint Dive into the research topics of 'Transnuclear CD8 T cells specific for the immunodominant epitope Gra6 lower acute-phase Toxoplasma gondii burden'. Together they form a unique fingerprint.

  • Cite this

    Sanecka, A., Yoshida, N., Dougan, S. K., Jackson, J., Shastri, N., Ploegh, H., Blanchard, N., & Frickel, E. M. (2016). Transnuclear CD8 T cells specific for the immunodominant epitope Gra6 lower acute-phase Toxoplasma gondii burden. Immunology, 149(3), 270-279. https://doi.org/10.1111/imm.12643