Transmission of S.aureus but not E.cloacae in an ICU

F. Perdreau-Remington, M. Webb, N. Huynh, Daniel Feikin, K. Hadley, J. Gerberding

Research output: Contribution to journalArticle

Abstract

Objective Using molecular typing of S.aureus (SAUR) and E.cloacae (ECLO), prospectively evaluate nosocomial bacterial pathogen source and spread in a population-based study of ICU patients. Methods: From 4/1/96 to 9/30/96, tracheal aspirates (TASP) from intubated ICU patients at SFGH were cultured at admission and regularly until extubation. All infecting/ colonizing isolates of SAUR and ECLO were also obtained from all ICU patients during the same time interval. SAUR isolates were genetically fingerprinted using PFGE of chromosomal ONA after restriction digest with Sma I; ECLO were similarly fingerprinted using Xba I. Results: 279 of 486 (57.4%) ICU patients were intubated. 94 patients had a TASP culture within 48 hrs of admission. 25 (26.5%) had SAUR in TASP at admission (index isolates); 23 SAUR index isolates were typed and 20 clones were found (3 were found twice). Of 18 patients with SAUR clinical isolates, 4 had SAUR identical to an index isolate. 3/25 patients with SAUR in TASP at admission developed SAUR infection with the same fingerprint (1 bacteremia/pnuemonia, 2 pneumonia). 8 patients acquired TASP SAUR colonization (not disease) in the ICU; 1 with an isolate identical to an index isolate. ECLO was isolated in TASP from 4 patients at admission; 13 patients acquired TASP ECLO (2 with identical strains that did not match the 4 index isolates). 5/17 (29%) patients with TASP ECLO developed nosocomial pneumonia most likely attributed to ECLO. Conclusions: Intubated ICU patients were often colonized with SAUR at admission and may be sources of person-to-person SAUR spread (5/23 typed index strains were later isolated from 1 or more ICU patients). SAUR disease was associated with colonization at admission but not later acquisition. Unlike SAUR, TASP ECLO was usually acquired after admission, acquisition was associated with infection, and did not appear to be readily transmitted person-to-person suggesting endogenous flora. Compared to antibiograms, molecular typing was useful in identifying otherwise occult person-to-person spread of pathogens in this ICU.

Original languageEnglish (US)
Pages (from-to)419
Number of pages1
JournalClinical Infectious Diseases
Volume25
Issue number2
StatePublished - 1997
Externally publishedYes

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Cloaca
Molecular Typing
Pneumonia
Patient Admission
Dermatoglyphics
Microbial Sensitivity Tests
Bacteremia
Infection

ASJC Scopus subject areas

  • Immunology

Cite this

Perdreau-Remington, F., Webb, M., Huynh, N., Feikin, D., Hadley, K., & Gerberding, J. (1997). Transmission of S.aureus but not E.cloacae in an ICU. Clinical Infectious Diseases, 25(2), 419.

Transmission of S.aureus but not E.cloacae in an ICU. / Perdreau-Remington, F.; Webb, M.; Huynh, N.; Feikin, Daniel; Hadley, K.; Gerberding, J.

In: Clinical Infectious Diseases, Vol. 25, No. 2, 1997, p. 419.

Research output: Contribution to journalArticle

Perdreau-Remington, F, Webb, M, Huynh, N, Feikin, D, Hadley, K & Gerberding, J 1997, 'Transmission of S.aureus but not E.cloacae in an ICU', Clinical Infectious Diseases, vol. 25, no. 2, pp. 419.
Perdreau-Remington F, Webb M, Huynh N, Feikin D, Hadley K, Gerberding J. Transmission of S.aureus but not E.cloacae in an ICU. Clinical Infectious Diseases. 1997;25(2):419.
Perdreau-Remington, F. ; Webb, M. ; Huynh, N. ; Feikin, Daniel ; Hadley, K. ; Gerberding, J. / Transmission of S.aureus but not E.cloacae in an ICU. In: Clinical Infectious Diseases. 1997 ; Vol. 25, No. 2. pp. 419.
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abstract = "Objective Using molecular typing of S.aureus (SAUR) and E.cloacae (ECLO), prospectively evaluate nosocomial bacterial pathogen source and spread in a population-based study of ICU patients. Methods: From 4/1/96 to 9/30/96, tracheal aspirates (TASP) from intubated ICU patients at SFGH were cultured at admission and regularly until extubation. All infecting/ colonizing isolates of SAUR and ECLO were also obtained from all ICU patients during the same time interval. SAUR isolates were genetically fingerprinted using PFGE of chromosomal ONA after restriction digest with Sma I; ECLO were similarly fingerprinted using Xba I. Results: 279 of 486 (57.4{\%}) ICU patients were intubated. 94 patients had a TASP culture within 48 hrs of admission. 25 (26.5{\%}) had SAUR in TASP at admission (index isolates); 23 SAUR index isolates were typed and 20 clones were found (3 were found twice). Of 18 patients with SAUR clinical isolates, 4 had SAUR identical to an index isolate. 3/25 patients with SAUR in TASP at admission developed SAUR infection with the same fingerprint (1 bacteremia/pnuemonia, 2 pneumonia). 8 patients acquired TASP SAUR colonization (not disease) in the ICU; 1 with an isolate identical to an index isolate. ECLO was isolated in TASP from 4 patients at admission; 13 patients acquired TASP ECLO (2 with identical strains that did not match the 4 index isolates). 5/17 (29{\%}) patients with TASP ECLO developed nosocomial pneumonia most likely attributed to ECLO. Conclusions: Intubated ICU patients were often colonized with SAUR at admission and may be sources of person-to-person SAUR spread (5/23 typed index strains were later isolated from 1 or more ICU patients). SAUR disease was associated with colonization at admission but not later acquisition. Unlike SAUR, TASP ECLO was usually acquired after admission, acquisition was associated with infection, and did not appear to be readily transmitted person-to-person suggesting endogenous flora. Compared to antibiograms, molecular typing was useful in identifying otherwise occult person-to-person spread of pathogens in this ICU.",
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AU - Gerberding, J.

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