Transmission of retroviral infection by transfusion of seronegative blood in nonhuman primates

Tamar Jehuda-Cohen, Jonathan Powell, Kenneth W. Sell, Francois Villinger, Ellen Lockwood, Harold M. McClure, A. Ahmed-Ansari

Research output: Contribution to journalArticle

Abstract

Techniques such as polyclonal B cell activation with pokeweed mitogen (PWM) and polymerase chain reaction (PCR) analysis have documented the existence of simian immunodeficiency virus (SIV)- and human immunodeficiency virus type t-seronegative but infected humans and nonhuman primates. To establish whether blood from such seronegative but PWM- and PCR-positive monkeys can transmit infection, naive macaques were transfused with whole blood (n = 2) or cultured cells and supernatant fluid (n = 2) from two seronegative but PWM- and PCR-positive sooty mangabeys. After transfusion, three of the four recipients seroconverted, and peripheral blood mononuclear cells from all four recipients secreted SIV-reactive antibodies upon polyclonal activation in vitro and were SIV-positive by PCR that used highly specific gag primer pairs and probe. In addition, CD8+ cells from all four recipients markedly inhibited replication of SIV in autologous cells in vitro. These data suggest caution in the sole use of serologic tests for the detection of retroviral infection aud document the ability of such blood samples to transmit infection.

Original languageEnglish (US)
Pages (from-to)1223-1228
Number of pages6
JournalJournal of Infectious Diseases
Volume163
Issue number6
StatePublished - Jun 1991
Externally publishedYes

Fingerprint

Simian Immunodeficiency Virus
Infectious Disease Transmission
Blood Transfusion
Pokeweed Mitogens
Primates
Polymerase Chain Reaction
Cercocebus atys
Infection
Macaca
Serologic Tests
Haplorhini
Cultured Cells
Blood Cells
B-Lymphocytes
HIV
Antibodies
In Vitro Techniques

ASJC Scopus subject areas

  • Public Health, Environmental and Occupational Health
  • Immunology

Cite this

Jehuda-Cohen, T., Powell, J., Sell, K. W., Villinger, F., Lockwood, E., McClure, H. M., & Ahmed-Ansari, A. (1991). Transmission of retroviral infection by transfusion of seronegative blood in nonhuman primates. Journal of Infectious Diseases, 163(6), 1223-1228.

Transmission of retroviral infection by transfusion of seronegative blood in nonhuman primates. / Jehuda-Cohen, Tamar; Powell, Jonathan; Sell, Kenneth W.; Villinger, Francois; Lockwood, Ellen; McClure, Harold M.; Ahmed-Ansari, A.

In: Journal of Infectious Diseases, Vol. 163, No. 6, 06.1991, p. 1223-1228.

Research output: Contribution to journalArticle

Jehuda-Cohen, T, Powell, J, Sell, KW, Villinger, F, Lockwood, E, McClure, HM & Ahmed-Ansari, A 1991, 'Transmission of retroviral infection by transfusion of seronegative blood in nonhuman primates', Journal of Infectious Diseases, vol. 163, no. 6, pp. 1223-1228.
Jehuda-Cohen T, Powell J, Sell KW, Villinger F, Lockwood E, McClure HM et al. Transmission of retroviral infection by transfusion of seronegative blood in nonhuman primates. Journal of Infectious Diseases. 1991 Jun;163(6):1223-1228.
Jehuda-Cohen, Tamar ; Powell, Jonathan ; Sell, Kenneth W. ; Villinger, Francois ; Lockwood, Ellen ; McClure, Harold M. ; Ahmed-Ansari, A. / Transmission of retroviral infection by transfusion of seronegative blood in nonhuman primates. In: Journal of Infectious Diseases. 1991 ; Vol. 163, No. 6. pp. 1223-1228.
@article{30c0f7ac59244a4bb8104a344bef022f,
title = "Transmission of retroviral infection by transfusion of seronegative blood in nonhuman primates",
abstract = "Techniques such as polyclonal B cell activation with pokeweed mitogen (PWM) and polymerase chain reaction (PCR) analysis have documented the existence of simian immunodeficiency virus (SIV)- and human immunodeficiency virus type t-seronegative but infected humans and nonhuman primates. To establish whether blood from such seronegative but PWM- and PCR-positive monkeys can transmit infection, naive macaques were transfused with whole blood (n = 2) or cultured cells and supernatant fluid (n = 2) from two seronegative but PWM- and PCR-positive sooty mangabeys. After transfusion, three of the four recipients seroconverted, and peripheral blood mononuclear cells from all four recipients secreted SIV-reactive antibodies upon polyclonal activation in vitro and were SIV-positive by PCR that used highly specific gag primer pairs and probe. In addition, CD8+ cells from all four recipients markedly inhibited replication of SIV in autologous cells in vitro. These data suggest caution in the sole use of serologic tests for the detection of retroviral infection aud document the ability of such blood samples to transmit infection.",
author = "Tamar Jehuda-Cohen and Jonathan Powell and Sell, {Kenneth W.} and Francois Villinger and Ellen Lockwood and McClure, {Harold M.} and A. Ahmed-Ansari",
year = "1991",
month = "6",
language = "English (US)",
volume = "163",
pages = "1223--1228",
journal = "Journal of Infectious Diseases",
issn = "0022-1899",
publisher = "Oxford University Press",
number = "6",

}

TY - JOUR

T1 - Transmission of retroviral infection by transfusion of seronegative blood in nonhuman primates

AU - Jehuda-Cohen, Tamar

AU - Powell, Jonathan

AU - Sell, Kenneth W.

AU - Villinger, Francois

AU - Lockwood, Ellen

AU - McClure, Harold M.

AU - Ahmed-Ansari, A.

PY - 1991/6

Y1 - 1991/6

N2 - Techniques such as polyclonal B cell activation with pokeweed mitogen (PWM) and polymerase chain reaction (PCR) analysis have documented the existence of simian immunodeficiency virus (SIV)- and human immunodeficiency virus type t-seronegative but infected humans and nonhuman primates. To establish whether blood from such seronegative but PWM- and PCR-positive monkeys can transmit infection, naive macaques were transfused with whole blood (n = 2) or cultured cells and supernatant fluid (n = 2) from two seronegative but PWM- and PCR-positive sooty mangabeys. After transfusion, three of the four recipients seroconverted, and peripheral blood mononuclear cells from all four recipients secreted SIV-reactive antibodies upon polyclonal activation in vitro and were SIV-positive by PCR that used highly specific gag primer pairs and probe. In addition, CD8+ cells from all four recipients markedly inhibited replication of SIV in autologous cells in vitro. These data suggest caution in the sole use of serologic tests for the detection of retroviral infection aud document the ability of such blood samples to transmit infection.

AB - Techniques such as polyclonal B cell activation with pokeweed mitogen (PWM) and polymerase chain reaction (PCR) analysis have documented the existence of simian immunodeficiency virus (SIV)- and human immunodeficiency virus type t-seronegative but infected humans and nonhuman primates. To establish whether blood from such seronegative but PWM- and PCR-positive monkeys can transmit infection, naive macaques were transfused with whole blood (n = 2) or cultured cells and supernatant fluid (n = 2) from two seronegative but PWM- and PCR-positive sooty mangabeys. After transfusion, three of the four recipients seroconverted, and peripheral blood mononuclear cells from all four recipients secreted SIV-reactive antibodies upon polyclonal activation in vitro and were SIV-positive by PCR that used highly specific gag primer pairs and probe. In addition, CD8+ cells from all four recipients markedly inhibited replication of SIV in autologous cells in vitro. These data suggest caution in the sole use of serologic tests for the detection of retroviral infection aud document the ability of such blood samples to transmit infection.

UR - http://www.scopus.com/inward/record.url?scp=0025946398&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=0025946398&partnerID=8YFLogxK

M3 - Article

C2 - 2037788

AN - SCOPUS:0025946398

VL - 163

SP - 1223

EP - 1228

JO - Journal of Infectious Diseases

JF - Journal of Infectious Diseases

SN - 0022-1899

IS - 6

ER -