Transmission of genetically diverse strains of HIV-1 in Pune, India

D. A. Gadkari, D. Moore, H. W. Sheppard, S. S. Kulkarni, S. M. Mehendale, R. C. Bollinger

Research output: Contribution to journalArticle

Abstract

Surveillance of the different HIV-1 subtypes has important implications for developing candidate vaccines and understanding the dynamics of HIV transmission in various populations. In this study, HIV-1 viral subtypes were determined for homologies in the V3-V5 region by heteroduplex mobility assay (HMA) in 46 patients with sexually transmitted diseases (STD) in Pune, India. Proviral DNA from peripheral blood mononuclear cells (PBMCs) from 20 recent sero-coverters and 26 HIV seropositive individuals were analyzed. Of the 46 samples analyzed, 44 (96%) were HIV-1 subtype C and one each of subtypes A and B. Further analyses revealed that 29 (66%) of the C subtype samples had maximum homology to the C3-Indian reference strain, while 15 (34%) were most homologous to the C2-Zambian strain. The C3 genotype prevailed in the majority (80%) of the seropositive individuals. Most of the C3 (Indian) strains were closely homologous to each other, while more nucleotide sequence divergence was seen in C2 samples. A higher quasispecies complexity was observed in the samples collected from seropositive individuals. These findings may have important implications for the design and testing of effective candidate HIV-1 vaccines for India.

Original languageEnglish (US)
Pages (from-to)1-9
Number of pages9
JournalIndian Journal of Medical Research
Volume107
Issue numberJAN.
StatePublished - Feb 24 1998

Keywords

  • HIV-1
  • India
  • Subtypes
  • Transmission

ASJC Scopus subject areas

  • Biochemistry, Genetics and Molecular Biology(all)

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  • Cite this

    Gadkari, D. A., Moore, D., Sheppard, H. W., Kulkarni, S. S., Mehendale, S. M., & Bollinger, R. C. (1998). Transmission of genetically diverse strains of HIV-1 in Pune, India. Indian Journal of Medical Research, 107(JAN.), 1-9.