Transmission of Chlamydia trachomatis among sex partners assessed by polymerase chain reaction

Raphael P Viscidi, Linda Bobo, Edward W. Hook, Thomas C Quinn

Research output: Contribution to journalArticle

Abstract

The prevalence of Chlamydia infection in 95 sex partners was determined by both polymerase chain reaction (PCR) and cell culture. Thirty-three (18%) of 186 specimens were positive by culture and 61 (33%) were positive by PCR-EIA. PCR was positive in 75% (21/28) of male partners of PCR-positive women compared with culture, which was positive in only 45% (9/19) of male partners of culture-positive women (P = .053). For female partners of infected men, the difference was less marked. PCR was positive in 58% (21/36) of female partners of infected men versus culture, which was positive in 56% (15/36) of female partners of culture-positive men. The correlation of PCR between partners and sequence analysis of Chlamydia DNA showing the same sequence from sex partners of 7 couples support the accuracy of the assay. These data suggest that PCR is more sensitive than culture for detection of Chlamydia trachomatis, particularly for male partners of infected women.

Original languageEnglish (US)
Pages (from-to)488-492
Number of pages5
JournalJournal of Infectious Diseases
Volume168
Issue number2
StatePublished - Aug 1993

Fingerprint

Chlamydia trachomatis
Polymerase Chain Reaction
Chlamydia Infections
Chlamydia
DNA Sequence Analysis
Cell Culture Techniques

ASJC Scopus subject areas

  • Immunology
  • Public Health, Environmental and Occupational Health

Cite this

Transmission of Chlamydia trachomatis among sex partners assessed by polymerase chain reaction. / Viscidi, Raphael P; Bobo, Linda; Hook, Edward W.; Quinn, Thomas C.

In: Journal of Infectious Diseases, Vol. 168, No. 2, 08.1993, p. 488-492.

Research output: Contribution to journalArticle

@article{3146a8893dd94e95b873e01033e1a24a,
title = "Transmission of Chlamydia trachomatis among sex partners assessed by polymerase chain reaction",
abstract = "The prevalence of Chlamydia infection in 95 sex partners was determined by both polymerase chain reaction (PCR) and cell culture. Thirty-three (18{\%}) of 186 specimens were positive by culture and 61 (33{\%}) were positive by PCR-EIA. PCR was positive in 75{\%} (21/28) of male partners of PCR-positive women compared with culture, which was positive in only 45{\%} (9/19) of male partners of culture-positive women (P = .053). For female partners of infected men, the difference was less marked. PCR was positive in 58{\%} (21/36) of female partners of infected men versus culture, which was positive in 56{\%} (15/36) of female partners of culture-positive men. The correlation of PCR between partners and sequence analysis of Chlamydia DNA showing the same sequence from sex partners of 7 couples support the accuracy of the assay. These data suggest that PCR is more sensitive than culture for detection of Chlamydia trachomatis, particularly for male partners of infected women.",
author = "Viscidi, {Raphael P} and Linda Bobo and Hook, {Edward W.} and Quinn, {Thomas C}",
year = "1993",
month = "8",
language = "English (US)",
volume = "168",
pages = "488--492",
journal = "Journal of Infectious Diseases",
issn = "0022-1899",
publisher = "Oxford University Press",
number = "2",

}

TY - JOUR

T1 - Transmission of Chlamydia trachomatis among sex partners assessed by polymerase chain reaction

AU - Viscidi, Raphael P

AU - Bobo, Linda

AU - Hook, Edward W.

AU - Quinn, Thomas C

PY - 1993/8

Y1 - 1993/8

N2 - The prevalence of Chlamydia infection in 95 sex partners was determined by both polymerase chain reaction (PCR) and cell culture. Thirty-three (18%) of 186 specimens were positive by culture and 61 (33%) were positive by PCR-EIA. PCR was positive in 75% (21/28) of male partners of PCR-positive women compared with culture, which was positive in only 45% (9/19) of male partners of culture-positive women (P = .053). For female partners of infected men, the difference was less marked. PCR was positive in 58% (21/36) of female partners of infected men versus culture, which was positive in 56% (15/36) of female partners of culture-positive men. The correlation of PCR between partners and sequence analysis of Chlamydia DNA showing the same sequence from sex partners of 7 couples support the accuracy of the assay. These data suggest that PCR is more sensitive than culture for detection of Chlamydia trachomatis, particularly for male partners of infected women.

AB - The prevalence of Chlamydia infection in 95 sex partners was determined by both polymerase chain reaction (PCR) and cell culture. Thirty-three (18%) of 186 specimens were positive by culture and 61 (33%) were positive by PCR-EIA. PCR was positive in 75% (21/28) of male partners of PCR-positive women compared with culture, which was positive in only 45% (9/19) of male partners of culture-positive women (P = .053). For female partners of infected men, the difference was less marked. PCR was positive in 58% (21/36) of female partners of infected men versus culture, which was positive in 56% (15/36) of female partners of culture-positive men. The correlation of PCR between partners and sequence analysis of Chlamydia DNA showing the same sequence from sex partners of 7 couples support the accuracy of the assay. These data suggest that PCR is more sensitive than culture for detection of Chlamydia trachomatis, particularly for male partners of infected women.

UR - http://www.scopus.com/inward/record.url?scp=0027261211&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=0027261211&partnerID=8YFLogxK

M3 - Article

C2 - 8335993

AN - SCOPUS:0027261211

VL - 168

SP - 488

EP - 492

JO - Journal of Infectious Diseases

JF - Journal of Infectious Diseases

SN - 0022-1899

IS - 2

ER -