Transmission of Chlamydia trachomatis among Sex Partners Assessed by Polymerase Chain Reaction

Raphael P. Viscidi, Linda Hobo, Edward W. Hook, Thomas C. Quinn

Research output: Contribution to journalArticlepeer-review

35 Scopus citations


The prevalence of Chlamydia infection in 95 sex partners was determined by both polymerase chain reaction (PCR) and cell culture. Thirty-three (18%) of 186 specimens were positive by culture and 61 (33%) were positive by PCR-EIA. PCR was positive in 75% (21/28) of male partners of PCR-positive women compared with culture, which was positive in only 45% (9/19) of male partners of culture-positive women (P =.053). For female partners of infected men, the difference was less marked. PCR was positive in 58% (21/36) of female partners of infected men versus culture, which was positive in 56% (15/36) offemale partners of culture-positive men. The correlation of PCR between partners and sequence analysis of Chlamydia DNA showing the same sequence from sex partners of 7 couples support the accuracy of the assay. These data suggest that PCR is more sensitive than culture for detection of Chlamydia trachomatis, particularly for male partners of infected women.

Original languageEnglish (US)
Pages (from-to)488-492
Number of pages5
JournalJournal of Infectious Diseases
Issue number2
StatePublished - Aug 1993

ASJC Scopus subject areas

  • Immunology and Allergy
  • Infectious Diseases


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