Translational repression restricts expression of the C. elegans Nanos homolog NOS-2 to the embryonic germline

Ingrid D'Agostino, Chris Merritt, Pei Lung Chen, Geraldine Seydoux, Kuppuswamy Subramaniam

Research output: Contribution to journalArticle

Abstract

Members of the nanos gene family are evolutionarily conserved regulators of germ cell development. In several organisms, Nanos protein expression is restricted to the primordial germ cells (PGCs) during early embryogenesis. Here, we investigate the regulation of the Caenorhabditis elegans nanos homolog nos-2. We find that the nos-2 RNA is translationally repressed. In the adult germline, translation of the nos-2 RNA is inhibited in growing oocytes, and this inhibition depends on a short stem loop in the nos-2 3′UTR. In embryos, nos-2 translation is repressed in early blastomeres, and this inhibition depends on a second region in the nos-2 3′UTR. nos-2 RNA is also degraded in somatic blastomeres by a process that is independent of translational repression and requires the CCCH finger proteins MEX-5 and MEX-6. Finally, the germ plasm component POS-1 activates nos-2 translation in the PGCs. A combination of translational repression, RNA degradation, and activation by germ plasm has also been implicated in the regulation of nanos homologs in Drosophila and zebrafish, suggesting the existence of conserved mechanisms to restrict Nanos expression to the germline.

Original languageEnglish (US)
Pages (from-to)244-252
Number of pages9
JournalDevelopmental Biology
Volume292
Issue number1
DOIs
StatePublished - Apr 1 2006

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Germ Cells
Blastomeres
RNA
RNA Stability
Caenorhabditis elegans
Zebrafish
Fingers
Drosophila
Oocytes
Embryonic Development
Proteins
Embryonic Structures
Genes

Keywords

  • Caenorhabditis elegans
  • CCCH finger
  • Germ cells
  • Nanos
  • RNA degradation
  • Translational control

ASJC Scopus subject areas

  • Developmental Biology

Cite this

Translational repression restricts expression of the C. elegans Nanos homolog NOS-2 to the embryonic germline. / D'Agostino, Ingrid; Merritt, Chris; Chen, Pei Lung; Seydoux, Geraldine; Subramaniam, Kuppuswamy.

In: Developmental Biology, Vol. 292, No. 1, 01.04.2006, p. 244-252.

Research output: Contribution to journalArticle

D'Agostino, Ingrid ; Merritt, Chris ; Chen, Pei Lung ; Seydoux, Geraldine ; Subramaniam, Kuppuswamy. / Translational repression restricts expression of the C. elegans Nanos homolog NOS-2 to the embryonic germline. In: Developmental Biology. 2006 ; Vol. 292, No. 1. pp. 244-252.
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