Translational profiles of alpha 1-, alpha 2-, and beta-globin messenger ribonucleic acids in human reticulocytes

Susan Eshleman, S. A. Liebhaber

Research output: Contribution to journalArticle

Abstract

In human reticulocytes, the critical balancing of α- and β-globin synthesis may be controlled in part by differential translation of the three major adult globin messenger RNAs (mRNAs), α1, α2, and β. In this study, we determined, as a parameter of translational efficiency, the relative ribosome loading of these three mRNAs. Using oligonucleotide probes specific for the α1- and α2-globin mRNAs, we find that these two mRNAs have identical translational profiles. Their distribution contrasts with that of β-globin mRNA, which is present on heavier polyribosomes and is less prevalent in pre-80S messenger ribonucleoprotein fractions. The relative distribution of α- vs. β-globin mRNA is consistent with more efficient β-globin translation. In contrast, the parallel distributions of α1- and α2-globin mRNAs suggests they are translated with equal efficiencies. Considering the relative concentrations of the two α-globin mRNAs in normal reticulocytes, this result of predicts a dominant role for the α2-globin locus in human α-globin expression.

Original languageEnglish (US)
Pages (from-to)1125-1129
Number of pages5
JournalJournal of Clinical Investigation
Volume78
Issue number4
StatePublished - 1986
Externally publishedYes

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alpha-Globins
beta-Globins
Globins
Reticulocytes
RNA
Messenger RNA
Polyribosomes
Oligonucleotide Probes
Ribosomes

ASJC Scopus subject areas

  • Medicine(all)

Cite this

Translational profiles of alpha 1-, alpha 2-, and beta-globin messenger ribonucleic acids in human reticulocytes. / Eshleman, Susan; Liebhaber, S. A.

In: Journal of Clinical Investigation, Vol. 78, No. 4, 1986, p. 1125-1129.

Research output: Contribution to journalArticle

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