Translational discrimination of ribosomal protein mRNAs in the early Drosophila embryo

Research output: Contribution to journalArticle

Abstract

Most Drosophila mRNAs are actively translated in the early embryo, with the exception of the poorly translated ribosomal protein (r-protein) mRNAs. Two possible mechanisms for this translational discrimination were tested: (1) Translation of r-protein mRNAs is discriminated against by the limited activity of translational initiation factors in the early embryo and (2) translation of r-protein mRNAs is repressed by trans-acting factors that reversibly bind these mRNAs. Exogenously provided initiation factors promoted partial recruitment of r-protein mRNAs into polysomes, suggesting that modulation of initiation factor activity may play a role in the translational discrimination of r-protein mRNAs during embryogenesis. No evidence for involvement of reversibly binding trans-acting factors was obtained, although there are limitations in the interpretation of the latter experiments.

Original languageEnglish (US)
Pages (from-to)338-343
Number of pages6
JournalDevelopmental Biology
Volume145
Issue number2
DOIs
StatePublished - 1991
Externally publishedYes

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Ribosomal Proteins
Drosophila
Embryonic Structures
Messenger RNA
Peptide Initiation Factors
Trans-Activators
Polyribosomes
Embryonic Development

ASJC Scopus subject areas

  • Developmental Biology

Cite this

Translational discrimination of ribosomal protein mRNAs in the early Drosophila embryo. / Hongo, Seiji; Jacobs-Lorena, Marcelo.

In: Developmental Biology, Vol. 145, No. 2, 1991, p. 338-343.

Research output: Contribution to journalArticle

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