TY - JOUR
T1 - Translational discrimination of ribosomal protein mRNAs in the early Drosophila embryo
AU - Hongo, Seiji
AU - Jacobs-Lorena, Marcelo
N1 - Funding Information:
We are indebted to Dr. W. Merrick for generously providing initiation factor preparations. We thank Drs. W. Merrick, R. Patel, P. Harte, and two anonymous reviewers for useful comments on the manuscript. This work was supported by Grant GM25327 from the National Institutes of Health.
PY - 1991/6
Y1 - 1991/6
N2 - Most Drosophila mRNAs are actively translated in the early embryo, with the exception of the poorly translated ribosomal protein (r-protein) mRNAs. Two possible mechanisms for this translational discrimination were tested: (1) Translation of r-protein mRNAs is discriminated against by the limited activity of translational initiation factors in the early embryo and (2) translation of r-protein mRNAs is repressed by trans-acting factors that reversibly bind these mRNAs. Exogenously provided initiation factors promoted partial recruitment of r-protein mRNAs into polysomes, suggesting that modulation of initiation factor activity may play a role in the translational discrimination of r-protein mRNAs during embryogenesis. No evidence for involvement of reversibly binding trans-acting factors was obtained, although there are limitations in the interpretation of the latter experiments.
AB - Most Drosophila mRNAs are actively translated in the early embryo, with the exception of the poorly translated ribosomal protein (r-protein) mRNAs. Two possible mechanisms for this translational discrimination were tested: (1) Translation of r-protein mRNAs is discriminated against by the limited activity of translational initiation factors in the early embryo and (2) translation of r-protein mRNAs is repressed by trans-acting factors that reversibly bind these mRNAs. Exogenously provided initiation factors promoted partial recruitment of r-protein mRNAs into polysomes, suggesting that modulation of initiation factor activity may play a role in the translational discrimination of r-protein mRNAs during embryogenesis. No evidence for involvement of reversibly binding trans-acting factors was obtained, although there are limitations in the interpretation of the latter experiments.
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U2 - 10.1016/0012-1606(91)90132-M
DO - 10.1016/0012-1606(91)90132-M
M3 - Article
C2 - 1904036
AN - SCOPUS:0025739148
SN - 0012-1606
VL - 145
SP - 338
EP - 343
JO - Developmental biology
JF - Developmental biology
IS - 2
ER -