Translation is required for regulation of histone mRNA degradation

Reed A. Graves, Niranjan Pandey, Nunta Chodchoy, William F. Marzluff

Research output: Contribution to journalArticle

Abstract

When DNA synthesis is inhibited, the mRNAs coding for the replication-dependent histone proteins are selectively destabilized. The histone genes have been altered and reintroduced into tk- mouse L cells by cotransfection with the herpesvirus thymidine kinase gene. Two features of the mRNA are necessary for regulation of degradation: first, the hairpin loop must be present at the 3′ end of the histone mRNA; and second, the histone mRNA must be capable of being translated to within 300 nucleotides of the 3′ end of the RNA. Polyadenylated histone mRNAs are stable, as are histone mRNAs that contain in-frame termination codons early in the coding region or 500 nucleotide 3′ untranslated regions with a normal hairpin loop at the 3′ end.

Original languageEnglish (US)
Pages (from-to)615-626
Number of pages12
JournalCell
Volume48
Issue number4
DOIs
StatePublished - Feb 27 1987
Externally publishedYes

Fingerprint

RNA Stability
Histones
Degradation
Messenger RNA
Nucleotides
Genes
Thymidine Kinase
Terminator Codon
Herpesviridae
3' Untranslated Regions
RNA
DNA
Proteins

ASJC Scopus subject areas

  • Cell Biology
  • Molecular Biology

Cite this

Translation is required for regulation of histone mRNA degradation. / Graves, Reed A.; Pandey, Niranjan; Chodchoy, Nunta; Marzluff, William F.

In: Cell, Vol. 48, No. 4, 27.02.1987, p. 615-626.

Research output: Contribution to journalArticle

Graves, Reed A. ; Pandey, Niranjan ; Chodchoy, Nunta ; Marzluff, William F. / Translation is required for regulation of histone mRNA degradation. In: Cell. 1987 ; Vol. 48, No. 4. pp. 615-626.
@article{096ad08c56514b05bfc38567cecd747c,
title = "Translation is required for regulation of histone mRNA degradation",
abstract = "When DNA synthesis is inhibited, the mRNAs coding for the replication-dependent histone proteins are selectively destabilized. The histone genes have been altered and reintroduced into tk- mouse L cells by cotransfection with the herpesvirus thymidine kinase gene. Two features of the mRNA are necessary for regulation of degradation: first, the hairpin loop must be present at the 3′ end of the histone mRNA; and second, the histone mRNA must be capable of being translated to within 300 nucleotides of the 3′ end of the RNA. Polyadenylated histone mRNAs are stable, as are histone mRNAs that contain in-frame termination codons early in the coding region or 500 nucleotide 3′ untranslated regions with a normal hairpin loop at the 3′ end.",
author = "Graves, {Reed A.} and Niranjan Pandey and Nunta Chodchoy and Marzluff, {William F.}",
year = "1987",
month = "2",
day = "27",
doi = "10.1016/0092-8674(87)90240-6",
language = "English (US)",
volume = "48",
pages = "615--626",
journal = "Cell",
issn = "0092-8674",
publisher = "Cell Press",
number = "4",

}

TY - JOUR

T1 - Translation is required for regulation of histone mRNA degradation

AU - Graves, Reed A.

AU - Pandey, Niranjan

AU - Chodchoy, Nunta

AU - Marzluff, William F.

PY - 1987/2/27

Y1 - 1987/2/27

N2 - When DNA synthesis is inhibited, the mRNAs coding for the replication-dependent histone proteins are selectively destabilized. The histone genes have been altered and reintroduced into tk- mouse L cells by cotransfection with the herpesvirus thymidine kinase gene. Two features of the mRNA are necessary for regulation of degradation: first, the hairpin loop must be present at the 3′ end of the histone mRNA; and second, the histone mRNA must be capable of being translated to within 300 nucleotides of the 3′ end of the RNA. Polyadenylated histone mRNAs are stable, as are histone mRNAs that contain in-frame termination codons early in the coding region or 500 nucleotide 3′ untranslated regions with a normal hairpin loop at the 3′ end.

AB - When DNA synthesis is inhibited, the mRNAs coding for the replication-dependent histone proteins are selectively destabilized. The histone genes have been altered and reintroduced into tk- mouse L cells by cotransfection with the herpesvirus thymidine kinase gene. Two features of the mRNA are necessary for regulation of degradation: first, the hairpin loop must be present at the 3′ end of the histone mRNA; and second, the histone mRNA must be capable of being translated to within 300 nucleotides of the 3′ end of the RNA. Polyadenylated histone mRNAs are stable, as are histone mRNAs that contain in-frame termination codons early in the coding region or 500 nucleotide 3′ untranslated regions with a normal hairpin loop at the 3′ end.

UR - http://www.scopus.com/inward/record.url?scp=0023666085&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=0023666085&partnerID=8YFLogxK

U2 - 10.1016/0092-8674(87)90240-6

DO - 10.1016/0092-8674(87)90240-6

M3 - Article

C2 - 3028643

AN - SCOPUS:0023666085

VL - 48

SP - 615

EP - 626

JO - Cell

JF - Cell

SN - 0092-8674

IS - 4

ER -