Abstract
In the present study we investigate the impact of highly stable coding region secondary structures on mRNA translation efficiency. By introducing antisense segments into the 3′ non-translated region of human α-globin mRNA we are able to synthesize a series of transcripts in which site-specific secondary structures are introduced without altering the primary structure of the 5′ non-translated region, the coding region, or the encoded protein product. Coding region duplexes in close proximity to the AUG initiation codon are found to inhibit translation severely to a degree equal to that of a duplex that extends into the 5′ non-translated region. In contrast, mRNAs containing duplexes positioned further 3′ in the coding region translate at levels that are significantly higher although are still below those of native α-globin mRNA. The primary determinant of translation inhibition by coding region duplexes appears to be the proximity of the duplex to the AUG initiation codon and reflects a parallel inhibition of monosome formation. These data demonstrate that extensive coding region secondary structures suppress translation to a minimal or to a substantial degree depending on their distance from the initiation codon.
Original language | English (US) |
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Pages (from-to) | 609-621 |
Number of pages | 13 |
Journal | Journal of molecular biology |
Volume | 226 |
Issue number | 3 |
DOIs | |
State | Published - Aug 5 1992 |
Externally published | Yes |
Keywords
- globin mRNA
- mRNA: gene expression
- secondary structure
- translation
ASJC Scopus subject areas
- Structural Biology
- Molecular Biology